Epigenetic modifications, including those on DNA and histones, have been shown to regulate cellular metabolism by controlling expression of enzymes involved in the corresponding metabolic pathways. In turn, metabolic flux influences epigenetic regulation by affecting the biosynthetic balance of enzyme cofactors or donors for certain chromatin modifications. Recently, non-enzymatic covalent modifications (NECMs) by chemically reactive metabolites have been reported to manipulate chromatin architecture and gene transcription through multiple mechanisms. Here, we summarize these recent advances in the identification and characterization of NECMs on nucleic acids, histones, and transcription factors, providing an additional mechanistic link between metabolism and epigenetics.
Methylglyoxal
(MGO) is a reactive dicarbonyl metabolite that modifies
histones in vivo and induces changes in chromatin
structure and function. Here we report the synthesis and application
of a chemical probe for investigating MGO-glycation. A two-step synthesis
of a Cu-click compatible alkynyl oxoaldehyde probe (AlkMGO) via sequential
Dess–Martin and Riley oxidations is presented. This synthesis
elevates the accessibility and utility of an important tool for tracking,
enriching, and studying MGO-glycation to aid in understanding its
underlying biochemical functions.
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