The key steps to develop bioremediation agent from indigenous group of hydrolytic bacteria include isolation of the bacteria from polluted site followed by evaluation on pathogenicity levels and interactions among the isolated strains. This study aimed to evaluate synergism and antagonism between non-pathogenic and pathogenic groups of hydrolytic bacteria isolated from liquid biomedical wastes of hospitals in Semarang. Interactions among indigenous, non-pathogenic, hydrolytic bacterial isolates i.e. Bacillus velezensis R1.3, B. amyloliquefaciens R1.6, B. amyloliquefaciens R1.14, B. velezensis R1.16, B. licheniformis R2.5, and B. amyloliquefaciens R2.9 and with 20 other pathogenic ones were evaluated using overlay and cross streak methods. Observation was based on the formation of inhibition zone as evidence of antagonistic interaction and the absence of this zone as proof of synergic interaction. As results, the 6 non-pathogenic isolates are all synergic among each other, yet antagonistic against most of the 20 pathogenic ones. As conclusion, the obtained 6 non-pathogenic isolates could be mixed as bacterial consortium and used as major component of bioremediation agent of liquid hospital waste.
This study aimed to develop bioremediation agent with bacterial cells as components for the treatment of biomedical wastes from two hospitals in Semarang City (Central Java), i.e. Roemani Muhammadiyah (coded R1) and Wongsonegoro (coded R2). Single isolates and consortium of indigenous hydrolytic bacteria characterized as multiple hydrolytic enzyme producers with low-to non-pathogenic properties obtained from previous study were tested for their degradation performance. The degradation performance test is necessary to formulate components of bacterial consortium as bioremediation agent. The tests were conducted on the selected bacteria as single isolate and as consortium. The six bacteria tested as single colonies and as consortium were Bacillus velezensis R1.3, B. amyloliquefaciens R1.6, B. amyloliquefaciens R1.14, B. velezensis R1.16, B. licheniformis R2.5, and B. amyloliquefaciens R2.9. Degradation performance on biomedical waste mainly containing organic matters was assessed based on water pollution parameters on 4.0-L samples. They included a control, 6 samples treated with bacteria as single colonies, a sample treated with bacteria as indigenous consortium and a sample treated with bacteria as mixed consortium. Parameters of wastewater pollution measured included COD (Chemical Oxygen Demand), BOD (Biological Oxygen Demand), TSS (Total Soluble Solid), NH3, and PO4. Next, encapsulation of a bacterial consortium as the best condition for degradation was also carried out using maltodextrin to allow storage and preservation of the bioremediation agent for longer period. The encapsulated product was visualized in SEM images to evaluate its quality. The results showed that a consortium comprising 4 indigenous bacterial isolates from R1 hospital could decrease BOD of biomedical wastewater by 85% and TSS by 43%. Those from R2 showed performance in reducing PO4 by 21%. This study demonstrated that compared to single isolates and mixed bacterial consortium tested, the indigenous hydrolytic bacterial consortium showed better ability in improving BOD and TSS of liquid biomedical waste of R1 hospital.
This study expects to decide the impact of occupation fulfillment and occupation devotion on hierarchical citizenship conduct in representatives of PT. Indosat Ooredoo Mobile Business Partner, Bengkulu City. the populace utilized in this study were all representatives of PT. Indosat Ooredoo Mobile Business Partner, Bengkulu City. the quantity of tests utilized upwards of 54 respondents. The information that has been gathered is handled utilizing the Classical Assumption Test Technique, Multiple Linear Regression Analysis Technique, Coefficient of Determination Analysis (R2), and Partial Test (t-test), Simultaneous Test (f-test). The aftereffects of this study show that somewhat the Job Satisfaction variable significantly affects the variable Organizational Citizenship Behavior, Job Loyalty significantly affects the variable Organizational Citizenship Behavior and Organizational Commitment significantly affects the variable Organizational Citizenship Behavior. While the F trial of Job Satisfaction, Job Loyalty and Organizational Commitment affects Organizational Citizenship Behavior at PT. Indosat Ooredoo Mobile Business Partner, Bengkulu City. Keywords: Job Satisfaction, Job Loyalty, Organizational Commitment and Organizational Citizenship Behavior.
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