BackgroundThe current coronavirus disease of 2019 (COVID-19) pandemic has caused widespread disease and death. Rapid increases in patient volumes have exposed weaknesses in healthcare systems and challenged our ability to provide optimal patient care and adequate safety measures to healthcare workers (HCWs).ObjectiveTo test the hypothesis that US neurologists were experiencing significant challenges with lack of personal protective equipment (PPE), rapid changes in practice and varying institutional protocols, we conducted this survey study.MethodsA 36-item survey was distributed to neurologists around the US through various media platforms.ResultsOver a one-week period, 567 responses were received. Of these, 56% practiced in academia. A total of 87% had access to PPE with 45% being asked to reuse PPE due to shortages. The pandemic caused rapid changes in practice, most notably a shift towards providing care by teleneurology, although a third experienced challenges in transitioning to this model. Wide variations were noted both in testing and in the guidance provided for the exposed, sick or vulnerable HCWs. Notably, 59% of respondents felt that their practices were doing what they could, although 56% did not feel safe taking care of patients.ConclusionsResults from our survey demonstrate significant variability in preparedness and responsiveness to the COVID-19 pandemic in neurology, impacted by region, health care setting and practice model. Practice guidelines from professional societies and other national entities are needed to improve protection for physicians and their patients, promote recommended practice changes during a pandemic, and optimize future preparedness for public health emergencies.

Tuberculosis (TB) is a slow growing, potentially debilitating disease that has plagued humanity for centuries and has claimed numerous lives across the globe. Concerted efforts by researchers have culminated in the development of various strategies to combat this malady. This review aims to raise awareness of the rapidly increasing incidences of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis, highlighting the significant modifications that were introduced in the TB treatment regimen over the past decade. A description of the role of pathogen–host immune mechanisms together with strategies for prevention of the disease is discussed. The struggle to develop novel drug therapies has continued in an effort to reduce the treatment duration, improve patient compliance and outcomes, and circumvent TB resistance mechanisms. Herein, we give an overview of the extensive medicinal chemistry efforts made during the past decade toward the discovery of new chemotypes, which are potentially active against TB-resistant strains.

Late embryogenesis abundant (LEA) proteins are a group of hydrophilic proteins, which accumulate in plants under varied stress conditions like drought, salinity, extreme temperatures and oxidative stress suggesting their role in the protection of plants against these stresses. A transcript derived fragment (TDF) corresponding to LEA gene, which got differentially expressed in wild peanut, Arachis diogoi against the late leaf spot pathogen, Phaeoisariopsis personata was used in this study. We have cloned its full length cDNA by RACE-PCR, which was designated as AdLEA. AdLEA belongs to the atypical Group 5C of LEA protein family as confirmed by sequence analysis. Group 5C LEA protein subfamily contains Pfam LEA_2 domain and is highly hydrophobic. In native conditions, expression of AdLEA was upregulated considerably upon hormonal and abiotic stress treatments emphasizing its role in abiotic stress tolerance. Subcellular localization studies showed that AdLEA protein is distributed in both nucleus and cytosol. Ectopic expression of AdLEA in tobacco resulted in enhanced tolerance of plants to dehydration, salinity and oxidative stress with the transgenic plants showing higher chlorophyll content and reduced lipid peroxidation as compared to wild type plants. Overexpressed AdLEA tobacco plants maintained better photosynthetic efficiency under drought conditions as demonstrated by chlorophyll fluorescence measurements. These plants showed enhanced transcript accumulation of some stress-responsive genes. Our study also elucidates that ROS levels were significantly reduced in leaves and stomatal guard cells of transgenic plants upon stress treatments. These results suggest that AdLEA confers multiple stress tolerance to plants, which make it a potential gene for genetic modification in plants.

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The aim of the present study was to assess the influence of phosphorus levels and phosphorus solubilizing fungi on yield and nutrient uptake by wheat. The dry matter production by wheat at tillering, ear emergence, and harvest was significantly higher with 90 kg P2O5 ha−1 and was at par with 60 kg P2O5 ha−1. Application of Aspergillus awamori gave the highest dry matter accumulation at tillering, ear emergence, and harvest stage of crop growth. Increasing levels of phosphorus increased the grain and straw yield significantly up to 60 kg P2O5 ha−1 of wheat crop. However, the maximum grain and straw yield were obtained at 90 kg P2O5 ha−1, which was at par with 60 kg P2O5 ha−1. The combined application of 60 kg P2O5 ha−1 with A. awamori recorded significantly higher grain and straw yield. Increasing level of P application (0 to 90 kg P2O5 ha−1) and inoculation with A. awamori and A. niger significantly increased uptake of N, P, and K in wheat at all stages of crop growth. The maximum nitrogen, phosphorus, and potassium uptake was recorded at harvest (grain and straw) followed by ear emergence and tillering when seeds were inoculated with Aspergillus awamori.

The havoc unleashed by COVID-19 pandemic has paved way for secondary ominous fungal infections like Mucormycosis. It is caused by a class of opportunistic pathogens from the order Mucorales. Fatality rates due to this contagious infection are extremely high. Numerous clinical manifestations result in damage to multiple organs subject to the patient’s underlying condition. Lack of a proper detection method and reliable treatment has made the management of this infection troublesome. Several reports studying the behavior pattern of Mucorales inside the host by modulation of its defense mechanisms have helped in understanding the pathogenesis of this angio-invasive infection. Many recent advances in diagnosis and treatment of this fungal infection have not been much beneficial. Therefore, there is a need to foster more viable strategies. This article summarizes current and imminent approaches that could aid effective management of these secondary infections in these times of global pandemic. It is foreseen that the development of newer antifungal drugs, antimicrobial peptides, and nanotechnology-based approaches for drug delivery would help combat this infection and curb its spread.

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A novel acetylated triterpene bisdesmoside saponin is elucidated as named Hederagenin 3-O-L-rhamnopyranosyl (3 → 1)-[2,4-O-diacetyl-L-arabinopyranosyl]-28-O-D-glucopyranosyl-(2 → 1) [3-O-acetyl-D-glucopyranosyl] ester (1) along with two known saponins, hederagenin 3-O-(-L-arabinopyranoside-(1 → 3)-L-rhamnopyranosyl-(1 → 2)-L-arabinopyranoside (2) and hederagenin 3-O-[-D-xylopyranosyl-(1 → 3)-L-rhamnopyranosyl-(1 → 2)-L-arabinopyranoside] (3), from the pericarps of Sapindus mukorossi. e structures of these saponins were characterized by means of chemical and spectral methods including advanced 2D NMR studies.