This study helps to predict the formula and structure of active molecules which can be used as drugs. This result also enhances the traditional usage of M. oleifera which possesses a number of bioactive compounds.
Purpose: To explore the hypotensive activity and chemical composition of Moringa oleifera Lam (Moringaceae) roots.
Methods: The fresh roots of M. oleifera was cut into small pieces and successively extracted with petroleum ether (PE) and dichloromethane (DC). PE extract was further divided into MRP and MRP -1. DC extract showed a thick mass during evaporation which was separated as MRDC -IN. The mother liquor left was divided into MRDC and MRDC -1. All residues were analyzed by gas chromatographymass spectroscopy (GC-MS) using ZB-5 column. Identification of each extract and fraction was based on comparison of their retention indices (RI), by co-injecting authentic compounds (7, 20.3 %) , stigmastan -3, 5, diene (24, 19.32 %), methyl 14-hydroxy-5-tetradecenoate (9, 19.22 %), 1 , 11 diphenyl undecane (47, 18.78 %) and cyclopentanyl hexadecane (39, 14.44 %) were the major constituents among the various hydrocarbons, fatty acids, esters, alcohols, aldehydes, isothiocyanate, aromatics, steroids, terphenyl and sulphur-containing
Traditionally, ivy leaf (Hedera helix) has been used for a large number of ailments. In the 19th century, water-based extract of young leaves was used for respiratory diseases. The aim of the current study was to quantify the triterpene saponin named hederacoside C of ivy leaf spray-dried extract of Hedera helix using high-performance liquid chromatography (HPLC) method coupled with UV-visible detector. The solvent system consisted of solvent A (phosphoric Acid 85%: acetonitrile: water with the ratio of 2: 140: 860) and solvent B (phosphoric acid 85%: acetonitrile with the ratio of 2:998). A gradient elution was used for separation at 205 nm at a flow rate of 1.5 mL/min. The separation was performed using a C18 column at a temperature of 40°C. The method was validated as per ICH guidelines for precision, accuracy, recovery, ruggedness, and robustness. The method was found to be precise, accurate, robust, and reproducible according to the guidelines of United States Pharmacopeia 2014 and European Pharmacopeia 8.0. The content of triterpene saponins was found to be 17.6%. Despite many studies reported on the method development and quantification of compounds of Hedera helix, there is insufficient work reported on the spray-dried extracts of this plant. This study quantifies the hederacoside C from the spray-dried extract of the plant by developing an accurate, cheap, robust, and precise method. The proposed method can be of significant usage in the pharmaceutical industry.
The objective of the present study was to determine the acute and subacute toxicity profile of a polyherbal formulation called “Goubion” in addition to the in vivo antihyperuricemic study using fructose-induced hyperuricemia. Goubion is a combination of Colchicum autumnale (tuber), Tribulus terresteris (fruit), Vitex negundo (leaves), Smilax chinensis (root), Glycyrrhiza glabra (root), and Curcuma amada (rhizome). The acute toxicity study revealed no signs of mortality and morbidity at a single dose of 2000 mg/kg. Similarly, the results of the subacute repeated dose toxicity study exhibited no signs of mortality at any of the doses. However, significant changes in hematological, biochemical, and renal parameters were recorded at the dose of 60 mg/kg. Antihyperuricemic activity was tested at the dose of 15 mg/kg and 20 mg/kg of Goubion, respectively against 5 mg/kg Allopurinol. Based on the antihyperuricemic study, we infer that the Goubion has a significant hypouricemic action, as it remarkably decreased the elevated uric acid levels. The results also suggest the potential inhibitory capability of Goubion on xanthine oxidase dehydrogenase might be the mechanism behind the hypouricemic effect.
Prescribing herbal remedies is now becoming common by many practitioners, who propose herbal treatments along with conventional medicine systems to treat various ailments considering their synergistic effects. Rosmarinic acid is a potent phenolic compound and caffeic acid derivative. It has many miraculous biological activities. Anti-allergic, anti-angiogenic, anti-depressant, anti-inflammatory, antitumor, anti-microbial activity, and antiviral effect of rosmarinic acid has already been documented in previous literature. It is widely present in many formulations because of its hepatoprotective and antioxidant effect. This study aimed to develop and validate a RP-HPLC method for the standardization of O. basilicum L. raw material and extracts by using rosmarinic acid as a marker. The study also aimed to quantify the rosmarinic acid content in the methanol extract of O. basilicum leaves. A simple and cost-effective High Pressure Liquid Chromatography (HPLC) method for determining rosmarinic acid in Ocimum basilicum plant extract has been developed and validated. Methanol-water-orthophosphoric acid in the ratio of 95:5:1 was used as the mobile phase using a C18 reversed-phase column for the HPLC method. Signals were detected at 254 nm. The rosmarinic acid content was found to be 4.2 mg/10 mg of the plant extract. The method is sensitive and reproducible and ideal for quick routine analysis and can be employed for detecting rosmarinic acid in other herbs. Despite the availability of literature on the medicinal properties of this plant and its chemical constituents, only a limited number of papers have been published on the determination of the rosmarinic acid in Ocimum basilicum plant using RP-HPLC. Moreover, this is the first report that quantifies rosmarinic acid in the plant extract by HPLC without using expensive columns and complex buffer systems that deteriorate the chromatographic columns and in turn, enhance the cost of the used process.
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