Cationic nanogels with site-selected functionality were designed for the delivery of nucleic acid payloads targeting numerous therapeutic applications. Functional cationic nanogels containing quaternized 2-(dimethylamino)ethyl methacrylate and a cross-linker with reducible disulfide moieties (qNG) were prepared by activators generated by electron transfer (AGET) atom transfer radical polymerization (ATRP) in an inverse miniemulsion. Polyplex formation between the qNG and nucleic acid exemplified by plasmid DNA (pDNA) and short interfering RNA (siRNA duplexes) were evaluated. The delivery of polyplexes was optimized for the delivery of pDNA and siRNA to the Drosophila Schneider 2 (S2) cell-line. The qNG/nucleic acid (i.e., siRNA and pDNA) polyplexes were found to be highly effective in their capabilities to deliver their respective payloads.
Deltas constitute complex depositional systems formed when a land-derived gravity-flow (carrying water and sediments) discharges into a marine or lacustrine standing body of water. However, the complexity of deltaic sedimentary environments has been oversimplified by geoscientists over the years, considering just littoral deltas as the unique possible type of delta in natural systems. Nevertheless, a rational analysis suggests that deltas can be much more complex. In fact, the characteristics of deltaic deposits will depend on a complex interplay between the bulk density of the incoming flow and the salinity of the receiving water body. This paper explores the natural conditions of deltaic sedimentation according to different density contrasts. The rational analysis of deltaic systems allows to recognize three main fields for deltaic sedimentation, corresponding to (1) hypopycnal (2) homopycnal and (3) hyperpycnal delta settings. The hypopycnal delta field represents the situation when the bulk density of the incoming flow is lower than the density of the water in the basin. According to the salinity of the receiving water body, three different types of hypopycnal littoral deltas are recognized: hypersaline littoral deltas (HSLD), marine littoral deltas (MLD), and brackish littoral deltas (BLD). The basin salinity will determine the capacity of the delta for producing effective buoyant plumes, and consequently the characteristics and extension of prodelta deposits. Homopycnal littoral deltas (HOLD) form when the density of the incoming flow is roughly similar to the density of the water in the receiving basin. This situation is typical of clean bedload-dominated rivers entering freshwater lakes. Delta front deposits are dominated by sediment avalanches. Typical fallout prodelta deposits are absent or poorly developed since no buoyant plumes are generated. Hyperpycnal deltas form when the bulk density of the incoming flow is higher than the density of the water in the receiving basin. The interaction between flow type, flow density (due to the concentration of suspended sediments) and basin salinity defines three types of deltas, corresponding to hyperpycnal littoral deltas (HLD), hyperpycnal subaqueous deltas (HSD), and hyperpycnal fan deltas (HFD). Hyperpycnal littoral deltas are low-gradient shallow-water deltas formed when dirty rivers enter into brackish or normal-salinity marine basins, typically in wave or tide-dominated epicontinental seas or brackish lakes. Hyperpycnal subaqueous deltas represent the most common type of hyperpycnal delta, with channels and lobes generated in marine and lacustrine settings during long-lasting sediment-laden river-flood discharges. Finally, hyperpycnal fan deltas are subaqueous delta systems generated on high-gradient lacustrine or marine settings by episodic high-density fluvial discharges.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.