A nonlinear forecast system for the sea surface temperature (SST) anomalies over the whole tropical Pacific has been developed using a multi-layer perceptron neural network approach, where sea level pressure and SST anomalies were used as predictors to predict the five leading SST principal components at lead times from 3 to 15 months. Relative to the linear regression (LR) models, the nonlinear (NL) models showed higher correlation skills and lower root mean square errors over most areas of the domain, especially over the far western Pacific (west of 155 degrees E) and the eastern equatorial Pacific off Peru at lead times longer than 3 months, with correlation skills enhanced by 0.10-0.14. Seasonal and decadal changes in the prediction skills in the NL and LR models were also studied.

Nonlinear projections of the tropical Pacific sea surface temperature anomalies (SSTAs) onto North American winter (November–March) surface air temperature (SAT) and precipitation anomalies have been performed using neural networks. During El Niño, the linear SAT response has positive anomalies centered over Alaska and western Canada opposing weaker negative anomalies centered over the southeastern United States. In contrast, the nonlinear SAT response, which is excited during both strong El Niño and strong La Niña, has negative anomalies centered over Alaska and northwestern Canada and positive anomalies over much of the United States and southern Canada. For precipitation, the linear response during El Niño has a positive anomaly area stretching from the east coast to the southwest coast of the United States and another positive area in northern Canada, in opposition to the negative anomaly area over much of southern Canada and northern United States, and another negative area over Alaska. In contrast, the nonlinear precipitation response, which is excited during both strong El Niño and strong La Niña, displays positive anomalies over much of the United States and southern Canada, with the main center on the west coast at around 45°N and a weak center along the southeast coast, and negative anomalies over northwestern Canada and Alaska. The nonlinear response accounts for about one-fourth and one-third as much variance as the linear response of the SAT and precipitation, respectively. A polynomial fit further verifies the nonlinear response of both the SAT and precipitation to be mainly a quadratic response to ENSO. Both the linear and nonlinear response patterns of the SAT and precipitation are basically consistent with the circulation anomalies (the 500-mb geopotential height anomalies), detected separately by nonlinear projection. A cross-validation test shows that including the nonlinear (quadratic) response can potentially contribute to additional forecast skill over North America.

[1] Neural network models are used to reveal the nonlinear winter atmospheric teleconnection patterns associated with the El Niño-Southern Oscillation (ENSO) and with the Arctic Oscillation (AO) over the N. Hemisphere. The nonlinear teleconnections (for surface air temperature, precipitation, sea level pressure and 500 hPa geopotential height) are found to relate quadratically to the ENSO and AO indices. Relative to linear teleconnections, nonlinear teleconections appear to propagate perturbations farther, into regions where classical linear teleconnections are insignificant.

Chlorogenic acid (CGA) is the primary constituent of Caulis , a Chinese herb used for the treatment of rheumatoid arthritis (RA). The present study aimed to investigate whether CGA was able to inhibit the proliferation of the fibroblast-like synoviocyte cell line (RSC-364), stimulated by interleukin (IL)-6, through inducing apoptosis. Following incubation with IL-6 or IL-6 and CGA, the cellular proliferation of RSC-364 cells was detected by MTT assay. The ratio of apoptosed cells were detected by flow cytometry. Western blot analysis was performed to observe protein expression levels of key molecules involved in the Janus-activated kinase/signal transducer and activator of transcription 3 (JAK/STAT) signaling pathway [phosphorylated (p)-STAT3, JAK1 and gp130] and the nuclear factor κB (NF-κB) signaling pathway [phosphorylated (p)-inhibitor of κB kinase subunit α/β and NF-κB p50). It was revealed that CGA was able to inhibit the inflammatory proliferation of RSC-364 cells mediated by IL-6 through inducing apoptosis. CGA was also able to suppress the expression levels of key molecules in the JAK/STAT and NF-κB signaling pathways, and inhibit the activation of these signaling pathways in the inflammatory response through IL-6-mediated signaling, thereby resulting in the inhibition of the inflammatory proliferation of synoviocytes. The present results indicated that CGA may have potential as a novel therapeutic agent for inhibiting inflammatory hyperplasia of the synovium through inducing synoviocyte apoptosis in patients with RA.

These findings suggest that both Sal B and Tan IIA have cardioprotective function in certain levels through multiple targets related with NO production, such as eNOS phosphorylation, L-arginine uptake and CAT expression, which may have major clinical implications.

A nonlinear projection of the tropical Pacific sea surface temperature anomalies (SSTA) onto the Northern Hemisphere winter 500‐mbar geopotential height (Z500) by a neural network reveals asymmetric atmospheric patterns associated with El Niño and La Niña. While the linear response of Z500 to tropical Pacific SSTA is largely confined over North Pacific and North America, the nonlinear response, mainly a quadratic response to the SSTA, reveals that regardless of the sign of the SSTA, positive Z500 anomalies are found over the central‐eastern North America, North Atlantic and western Europe, and negative Z500 anomalies over the west coast of North America, Scandinavia and eastern Europe—consistent with the positive Pacific‐North America (PNA) teleconnection pattern and the positive North Atlantic Oscillation (NAO) pattern being excited.

Wenxin-Keli (WXKL) is a Chinese herbal compound reported to be of benefit in the treatment of cardiac arrhythmia, cardiac inflammation, and heart failure. Amiodarone is a noncompetitive inhibitor of the α- and β-adrenergic receptors and prevents calcium influx in the slow-response cells of the sinoatrial and atrioventricular nodes. Overexpression of Ca2+/calmodulin-dependent protein kinase II (CaMKII) in transgenic mice results in heart failure and arrhythmias. We hypothesised that administration of WXKL and amiodarone can reduce the incidence of arrhythmias by regulating CaMKII signal transduction. A total of 100 healthy Sprague Dawley rats were used in the study. The rats were randomly divided into four groups (a sham group, a myocardial infarction (MI) group, a WXKL-treated group, and an amiodarone-treated group). A myocardial infarction model was established in these rats by ligating the left anterior descending coronary artery for 4 weeks. Western blotting was used to assess CaMKII, p-CaMKII (Thr-286), PLB, p-PLB (Thr-17), RYR2, and FK binding protein 12.6 (FKBP12.6) levels. The Ca2+ content in the sarcoplasmic reticulum (SR) and the calcium transient amplitude were studied by confocal imaging using the fluorescent indicator Fura-4. In conclusion, WXKL may inhibit heart failure and cardiac arrhythmias by regulating the CaMKII signal transduction pathway similar to amiodarone.

Objective. We investigated the effects of WXKL on the action potential (AP) and the L-type calcium current (I Ca-L) in normal and hypertrophied myocytes. Methods. Forty male rats were randomly divided into two groups: the control group and the transverse aortic constriction- (TAC-) induced heart failure group. Cardiac hypertrophy was induced by TAC surgery, whereas the control group underwent a sham operation. Eight weeks after surgery, single cardiac ventricular myocytes were isolated from the hearts of the rats. The APs and I Ca-L were recorded using the whole-cell patch clamp technique. Results. The action potential duration (APD) of the TAC group was prolonged compared with the control group and was markedly shortened by WXKL treatment in a dose-dependent manner. The current densities of the I Ca-L in the TAC group treated with 5 g/L WXKL were significantly decreased compared with the TAC group. We also determined the effect of WXKL on the gating mechanism of the I Ca-L in the TAC group. We found that WXKL decreased the I Ca-L by accelerating the inactivation of the channels and delaying the recovery time from inactivation. Conclusions. The results suggest that WXKL affects the AP and blocked the I Ca-L, which ultimately resulted in the treatment of arrhythmias.