Electro-acupuncture at Zusanli and Guanyuan could improve clinical curative effect in patients with sepsis, which might be achieved by regulation of the immune system.
The long non-coding RNA (lncRNA) BANCR is a well-studied lncRNA that serves pivotal roles in various malignancies; however, to the best of our knowledge, its involvement in polycystic ovary syndrome (PCOS) remains unknown. In the present study, the expression levels of lncRNA BANCR were detected in granulosa cells (GCs) from patients with PCOS and non-PCOS patients undergoing in vitro fertilization by reverse transcription-quantitative polymerase chain reaction. Subsequently, GCs and the KGN human granulosa-like tumor cell line were treated with insulin, and BANCR expression was detected. KGN cells were also transfected with a BANCR expression vector, after which, cell proliferation, apoptosis and the expression levels of pro-apoptotic B-cell lymphoma 2-associated X protein (Bax) and p53 were detected by Cell Counting kit-8 assay, MTT assay and western blotting, respectively. The results revealed that the expression levels of lncRNA BANCR in GCs were significantly higher in patients with PCOS compared with in non-PCOS patients. In addition, insulin treatment significantly upregulated the expression of BANCR in GCs and KGN cells. Transfection with the BANCR expression vector significantly inhibited proliferation and promoted apoptosis of KGN cells, and significantly promoted the expression levels of pro-apoptotic Bax and p53. Therefore, it may be concluded that lncRNA BANCR participates in PCOS by promoting cell apoptosis through the upregulation of Bax and p53.
Background Enteral nutrition is a major pathway of nutrition for patients requiring critical care. However, it remains unclear whether intermittent or continuous feeding is the better approach, especially after nasogastric enteral nutrition via a gastric tube. Therefore, this randomized controlled clinical study was designed to observe the effects of different methods on critically ill patients. Methods Different Feeding Methods on Gastrointestinal Function of Critical patients (DFM-GFC) is a randomized clinical study that will be performed to assess the effects of three feeding methods on critically ill patients. A total of 90 critically ill patients will be equally randomized into three groups: continuous feeding, cyclic feeding, and intermittent feeding. The patients will be administered a gastrointestinal nutrition preparation over 24 h via a gastric tube or over 16 h via an intermittent pump. The primary outcome is the mean duration (days) to reach the caloric goal in each group. Secondary outcomes include the rate of onset of gastric residual, abdominal pressure, the rate of onset pneumonia, and the proportion of individuals achieving the caloric goal. Additionally, the length of intensive care unit (ICU) stay and mortality rate at 28 days post-enrolment will be evaluated. Discussion This study will observe the effects of different feeding methods on various parameters, such as the energy target and gastrointestinal motility, in critically ill patients to improve quality of life and reduce the case fatality rate. The purpose of this study is to explore whether there is a more effective, safer and cost-efficient feeding method for the clinical treatment of critically ill patients. Trial registration ID: NCT04224883, ClinicalTrials.gov, registered January 9, 2020
Background: Enteral nutrition is a major pathway of nutrition for patients requiring critical care. However, whether intermittent or continuous feeding is better is not yet known clearly, especially after nasogastric enteral nutrition via gastric tube. Therefore, this randomized controlled clinical study was designed to observe the effects of different methods on critically ill patients.Methods: The different feeding method on gastrointestinal function of critical patients (DFM-GFC) was a randomized, single-blind, clinical study assessing the effects of three feeding methods on critically ill patients. A total of 90 critical patients were equally randomized to three groups: continuous feeding, cycling feeding, and intermittent feeding. The patients were pumped with gastrointestinal nutrition preparation via gastric tube in 24 h or in 16 h via intermittent pump. The primary outcome is the mean duration that reached to the caloric goal in every group. The secondary outcome included the rate of onset of gastric residua, abdominal pressure, the rate of onset pneumonia, and the proportion of individuals achieving the caloric goal. Also, the length of intensive care unit (ICU) stay and mortality rate at 28 days post-enrollment was evaluated.Discussion: This study observes the effects of different feeding methods on parameters, such as energy target and gastrointestinal motility in critically ill patients, in order to improve the prognosis of, quality of life and reduce the case fatality rate.Trial registration: ClinicalTrials.gov ID: NCT04224883. Registered on January 9, 2020.
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