In order to study the mechanism of the effect of heparin on apoptosis in carcinoma cells, the nasopharyngeal carcinoma cell line CNE2 was used to identify the effect of heparin on apoptosis associated with the expression of c-myc, bax, bcl-2 proteins by use of Hoechst 33258 staining, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), agarose gel electrophoresis, and flow cytometry, as well as Western blot analysis. The results showed that heparin induced apoptosis of CNE2 cells including the morphologic changes such as reduction in the volume, and the nuclear chromatin condensation, as well as the "ladder pattern" revealed by agarose gel electrophoresis of DNA in a concentration-dependent manner. The number of TUNEL-positive cells was dramatically increased to 33.6+/-1.2% from 2.8+/-0.3% by treatment with heparin in different concentrations (10 to approximately 40 kU/L). The apoptotic index was increased to 32.5% from 3.5% by detecting SubG1 peaks on flow cytometry. Western blot analysis showed that levels of bcl-2, bax and c-myc were significantly overexpressed by treatment with the increase of heparin concentrations. These results suggest that heparin induces apoptosis of CNE2 cells, which may be regulated by differential expression of apoptosis-related genes.
Based on generalized non-Newtonian fluid with seven parameters Cross-WLF viscosity model and modified 2-domain Tait model, the injection process of short glass fiber-reinforced polypropylene is simulated with a true 3D model. The effects of gate location on clamp force, fiber orientation and parts deformation are investigated. The results show that: (1) Clamp force of the 3rd scheme that gate location is set on the center of long-side is the largest and that of the 1st scheme that gate location is set on the center of top is the smallest. (2)Because of shear stress, in the direction of thickness, the distributions of fiber orientation are layered orderly, namely, the degree of fiber orientation in core layer is the lowest, but that in subsurface layer is the highest. While perpendicular to the shear flow, the layering distribution isn’t obvious. (3) Compared the three schemes in respect of clamp force and parts deflection, the 3rd scheme is the poorest, which should not be adopted as can as possible, while the 1st scheme and 2nd scheme are reasonable.
Based on generalized non-Newtonian fluid with seven parameters Cross-WLF viscosity model and modified 2-double Tait model, the numerical simulation was carried out for the short glass fiber reinforced PP injection molding process of rectangular part. The influence of main process parameters on fiber orientation is investigated. The results show that fiber orientation can be generally divided into three-regional layers in injection molding, that is outer-surface, subsurface and core layer. The degree of fiber orientation in subsurface layer is the highest and that in core layer is the lowest. The influence of fibers interaction coefficient (Ci) and fibers aspect ratio (re) on fiber orientation is significant. There is obvious difference between simulation results and practical results without consideration of Ci. The effect of melt temperature, mold temperature and cooling tubes number on fiber orientation isn’t obvious.
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