BackgroundBacterial resistance to antibiotics is increasing worldwide. Antibiotic-resistant strains can lead to serious problems regarding treatment of infection. Carbapenem antibiotics are the final treatment option for infections caused by serious and life-threatening multidrug-resistant gram-negative bacteria. Therefore, an understanding of carbapenem resistance is important for infection control. In the study described herein, the phenotypic and genotypic features of carbapenem-resistant Enterobacteriaceae strains isolated in our hospital were evaluated.MethodsIn total, 43 carbapenem-resistant strains were included in this study. Sensitivity to antibiotics was determined using the VITEK®2 system. The modified Hodge test (MHT) and metallo-β-lactamase (MBL) antimicrobial gradient test were performed for phenotypic identification. Resistance genes IMP, VIM, KPC, NDM-1, and OXA-48 were amplified by multiplex PCR.ResultsThe OXA-48 gene was detected in seven strains, and the NDM-1 gene in one strain. No resistance genes were detected in the remainder of strains. A significant correlation was observed between the MHT test and OXA-48 positivity, and between the MBL antimicrobial gradient test and positivity for resistance genes (p < 0.05).ConclusionThe finding of one NDM-1-positive isolate in this study indicates that carbapenem resistance is spreading in Turkey. Carbapenem resistance spreads rapidly and causes challenges in treatment, and results in high mortality/morbidity rates. Therefore, is necessary to determine carbapenem resistance in Enterobacteriaceae isolates and to take essential infection control precautions to avoid spread of this resistance.
Mock trial is an activity commonly used both in and out of the class in citizenship education. Mock trial is a simulation technique rooted in experiential learning. This study aimed to evaluate the Mock trial method used by a primary school teacher in Turkey to solve the problems faced during lessons. Research Methods: This research is designed as an intrinsic case study, which involves the use of Mock trials to solve the classroom problems. Participants were a classroom teacher and 20 fourth grade students attending her class. Research data were analyzed using inductive thematic analysis. Findings: Mock trial procedure was conducted during the last 15 minutes of the lessons at one-totwo-month intervals. The problem to be handled in the Mock trial is decided by the consensus of the
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.