This study was undertaken to investigate and assess trace metal (Cd, Pb, Cr, and Hg) concentrations in the Cystoseira compressa algae from the coastal city of Bou Ismaïl (Algeria). Spatial and temporal variations in the concentrations of these heavy metals were studied in the following three sampling sites: site 1 and site 2 were major wastewater discharge zones and site 3 was located close to areas with high industrial activity. Seawater, sediments and algae in the wastewater from the three sites were submitted to physico-chemical analyses to determine the concentrations of heavy metals and the level of pollution in the region. The results revealed that site 1 (designed as desalination) and site 2 (designed as industrial waste) were non-compliant, which was attributed to heavy loads of heavy metals and wastewater discharged by industrial activity and absence of regular treatment. Site 3 (designed as cove koali), on the other hand, was noted to represent a reference site. Overall, the results provided evidence for the heavy metal bioaccumulation of algae from the genus Cystoseira and their efficiency for use as biomarkers of pollution in coastal areas.
Rabbit immunoglobulin G (IgG) was subjected to affinity chromatography on a column of jacalin-Sepharose 4B. While the majority of IgG molecules did not bind, a small fraction, representing about 25% of the total IgG applied, bound to jacalin-Sepharose 4B. The binding of rabbit IgG to jacalin was further evidenced by ELISA performed on jacalin coated microtitre plates. While the jacalin-retained IgG fraction displayed strong binding, the unretained fraction did not demonstrate any detectable binding. Upon SDS-PAGE, both the jacalin retained and unretained rabbit IgG fractions displayed identical protein profiles. Upon protein blotting it was demonstrated that jacalin binding sites were located only on the heavy chain of IgG. These results suggest that rabbit IgG molecules are heterogeneous with respect to their glycosylation patterns. A small fraction of rabbit IgG molecules binds jacalin and the process is probably mediated through O-linked oligosaccharides present on the heavy chain of IgG.
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