Aluminum TLC plates precoated with silica gel 60 GF 254 (20×20cm), (Merck, Darmstadt, Germany). c. Chromatographic tank (25×25×9cm). Materials and reagents Pure standard Standard PHZ (certified to contain 99.94%) and TMP (certified to contain 99.91%) were kindly supplied by kahira for pharmaceuticals, Cairo, Egypt.
Objective: This study aimed to develop three simple UV spectrophotometric methods for determination of pravastatin sodium in the presence of pioglitazone hydrochloride without previous separation.
Methods:Manipulating ratio spectra were developed for the interested methods. The first method is the ratio subtraction using 16 µg/mL of pioglitazone hydrochloride as a divisor. The second method is an amplitude modulation using the normalized spectrum of pioglitazone hydrochloride as a divisor (1 µg/mL) the peak amplitudes of ratio spectra were measured at isoabsorptivity point 251.4 nm. The third method is the ratio difference using 14 µg/mL of pioglitazone hydrochloride as a divisor, where the peak amplitudes of ratio spectra were measured at 240 and 246.4 nm.
The results and discussion:The calibration curve is a linear over the concentration range of 2-20 µg/mL, the proposed methods were validated according to International Conference on Harmonization (ICH) guidelines and successfully applied for the determination of pravastatin sodium in the presence of pioglitazone hydrochloride in their combined pharmaceutical formulation, Pravazon® capsules with high sensitivity.
Conclusion:The proposed three methods are simple, rapid, economical, accurate and precise for determination of pravastatin sodium in the presence of pioglitazone hydrochloride without previous separation.
Objectives: A simple, accurate, selective and sensitive densitometric method was developed for the determination of ezetimibe in the presence of its alkaline degradation product. Methods: TLC-densitometric separation of ezetimibe from its degradation products was carried out on silica gel plates using ethyl acetate: n-hexane (2:1 v/v) as a developing system. This method depends on quantitative densitometric evaluation of ezetimibe at 230 nm over a concentration range of 1-8 µg/spot. Ezetimibe and its alkaline degradation product were resolved with Rf values of 0.49 and 0.68 respectively. Results: The proposed method has been successfully applied to the analysis of ezetimibe in pharmaceutical dosage form without interference from additives and the results were statistically compared with the reported method. Conclusion: TLC-densitometric technique has provided a simple, straightforward method for separating ezetimibe and its alkaline degradation product simultaneously.
Objective: This study aimed to develop three simple UV spectrophotometric methods for determination of pravastatin sodium in the presence of pioglitazone hydrochloride without previous separation. Methods: Ratio spectra were developed for the interested methods. The first method is the ratio subtraction using 16µg/mL of pioglitazone hydrochloride as a divisor. The second method is an amplitude modulation using the normalized spectrum of pioglitazone hydrochloride as a divisor (1µg/mL) the peak amplitudes of ratio spectra were measured at isoabsorptivity point 251.4nm. The third method is the ratio difference using 14µg/mL of pioglitazone hydrochloride as a divisor, where the peak amplitudes of ratio spectra were measured at 240 and 246.4nm. Results and discussion: The calibration curve is linear over the concentration range of 2-20µg/mL, the proposed methods were validated according to International Conference on Harmonization (ICH) guidelines and successfully applied for the determination of pravastatin sodium in the presence of pioglitazone hydrochloride in their combined pharmaceutical formulation, Pravazon® capsules with high sensitivity. Conclusion: The proposed three methods are simple, rapid, economical, accurate and precise for determination of pravastatin sodium in the presence of pioglitazone hydrochloride without previous separation.
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