The aim of this research to determine the concentration of flavonoid and anthocyanin compounds in sappan wood extract (Caesalpinia sappan L.), as well as to determine the concentration of inhibitory power against the growth of Vibrio cholerae. This experimental design study uses 5 kind of concentration, which are 20%, 40%, 60%, 80%, 100%. The analysis were repeated 3 times and resulting in 15 experimental units. The data were presented in descriptive statistic with tables, drawings and discussed. The results showed that the extract of sappan wood contained flavonoid compounds of 6,02%, anthocyanin compounds of 2,43% and was able to inhibit the growth of Vibrio cholerae with optimal concentration at a concentration of 80% with an average 20,1 mm and very strong inhibition categories.

This study aims to determine the Minimum Inhibitory Consentration (MIC) of andaliman fruit extract (Zanthoxylum acanthopodium DC) in ethyl acetate against the growth of Escherichia coli bacteria. The design of this study used an experimental method with 10 extract concentrations for MIC testing, namely 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% with 2 repetitions so that they were obtained 20 experimental units. The variables observed were the formation of clear zones, namely the inhibition produced by the presence of antimicrobial activity from andaliman fruit extract in ethyl acetate against the growth of Escherichia coli. Data from research results are presented in the form of tables, figure and discussed descriptively. Andaliman fruit extracts in ethyl acetate have varying inhibitory forces, namely at the concentrations of concentrations of 10% to 20% was 0.65 mm to 3.15 mm with weak inhibition categories, concentrations of 30% to 50% was 5.25 mm to 6, 60 mm in the moderate inhibition category and concentrations of 60% to 100% showed strong inhibition categories with inhibition diameters of 7.20 mm to 9.60 mm. Minimum Inhibitory Concentration (MIC) for the growth of Escherichia coli was at the concentration of 60% with a clear zone diameter of 7.2 mm with a strong category.     Keywords : Andaliman fruit, Escherichia coli, antibacterial.

IntroductionR. oligosporus is a fungus of the family Mucoraceae and is a widely used starter culture for the production of tempeh at home and industrially. As the mold grows it produces fluffy, white mycelia, binding the beans together to create an edible "cake" of partly catabolized soybeans. The domestication of the microbe is thought to have occurred in Indonesia several centuries ago (

This study aims to determine the levels of tannin and flavonoid compounds, as well as to determine the concentration of the extract of star anise (Illicium verum Hook.f) was most appropriate by used aquades solvent against the growth of Staphylococcus aureus. This research used experimental method with 5 kind of concentration which are 20%, 40%, 60%, 80% and 100%, each treatment was repeated 3 times to obtain 15 experimental units. The observed variables were quantitative testing of flavonoid and tannin compounds and create an inhibition zone produced by antimicrobial activity of the extract of star anise on Staphylococcus aureus. The data of this research are presented in the form of tables, figures and analyzed descriptively. The results showed that the extract contained flavonoid compounds of 0.106% and tannins of 1.018%. The extract of star anise can inhibit Staphylococcus aureus at a concentration of 40% with an average 7.03 mm inhibition zone.

This research aims to find out the effect of different types of substrates on the growth of Balinese local isolate Rhizopus oligosporus DPO2 in the making of tempeh yeast and to identify the right type of substrate for the growth of R. oligosporus DP02 in making tempeh yeast. This was an experimental research that was analyzed descriptively with type of substrates as the main factor that consists of 5 different flours including: rice, tapioca, corn, wheat and soybeans. Each treatment was repeated 3 times resulting 15 experimental units. The results showed that different types of substrate produced tempe yeast which has R. oligosporus DP02 Bali with dense to very dense mycelium, dry weight of mycelium 0.49 - 0.58 g, total mold 6.97 - 7.77 log CFU / g and water content. 4.47 - 6.01%. Corn flour produces tempeh yeast with the highest growth of R. oligosporus DP02 Bali, with the following characteristics: very dense mycelium, mycelium dry weight 0.58 g, total mold 7.77 log CFU / g and water content 4.55%.

The study aimed to apply various lactic acid bacteria (LAB) inoculum powder in fermentation of urutan (Balinese sausage) and gather information about the physicochemical characteristic such as the total protein content, fat content, pH, moisture content and soluble protein profile of fermented urutan. The results showed that the fermentation urutan with the LAB inoculum powder and conditioning process (192 h) have the highest protein content (33,91%), the lowest fat content (22,57%), the lowest pH (4,57) and the lowest moisture content (36,86%) indicated that the fermented urutan suitable according to quality standard required of meat sausage.

This research was aimed to determine the effect type of “ragi” on the fermentation process of cocoa and to determine type of “ragi” that can produce vinegar with the best characteristics. This research used Randomized Block Design (RBD) with four types treatment of “ragi”, these are: without the addition of “ragi”, addition of bread yeast, addition of “tape ragi”, and addition of combination “ragi”. “Ragi” additions were carried out as much as 1% in each treatment. This research was repeated three times, resulting in 12 experimental units. The data was analyzed with Anova test and continued with Duncan test if the treatment had a significant effect (P<0.05) on the observed variable. This research showed that the treatment fermentation addition of tape ragi was the best treatment with characteristic 5.05 pH, 0.88% total acid, 3.33% total soluble solids, 0.59% total sugar and 0% alcohol. Keyword: cocoa, ragi, vinegar, fermentation

The aim of this research was to determine the content of flavonoid and antibacterial activity of ethanol extract of sintrong leaf (Crassocephalum crepidiodes) on Bacillus cereus. This research used a Completely Randomized Design (CRD) with treatment concentrations extract which were 20%, 40%, 60%, 80%, and 100% with three replications and resulting in 15 experimental units. Data were presented with tables and pictures and analyzed in descriptive statistic. Ethanol extract of sintrong leaf contained flavonoid compounds of 1,75% and was able to inhibit the growth of Bacillus cereus at a concentration of 20% with an average 12,3 mm and strong inhibition categories, also has a bacteriostatic character with percentage range death of Bacillus cereus 80,9% - 93,7%. Keywords : flavonoid, sintrong leaf, antibacterial, bacillus cereus.