Vernalization-induced flowering is an effect of the epigenetic regulation of gene expression through DNA methylation and histone modifications. Vernalizationmediated silencing of a floral repressor through histone modifications was shown in Arabidopsis thaliana. However, for Brassica napus L., the mechanism underlying vernalization is unclear, and the roles of DNA methylation and histone modifications have not been established. This study revealed the profiles of changes in the DNA methylation state during vernalization (after 14, 35, 56 days) and the subsequent growth in long-or short-day photoperiods (after 2, 7, 14 days) in the winter and spring rapeseed using TLC and MSAP techniques. TLC analysis showed a significant decrease in the amount of 5-methylcytosine (m 5 C) in genomic DNA in both cultivars at the beginning of vernalization, but upon its termination, the winter rape showed a reduced level of m 5 C contrary to a significantly increased level in the spring rape. MSAP analysis revealed that winter and spring rapeseed differed in the MSAP loci which were demethylated/methylated in the course of the experiment and presented diverse profiles of changes in the methylation state. The winter rape showed permanent demethylations at 69.2 % of MSAP loci in the course of vernalization that were mostly preserved upon its termination. The spring rape showed similar numbers of demethylations and methylations that were mainly transient. The study provides evidence of the role of DNA methylation in vernalization for rapeseed and for the significant prevalence of demethylations at the beginning of vernalization, which is necessary for the transition to reproductive growth.
The infection capacity of Potato virus Y (PVY, genus Potyvirus) for wild-living plants, commonly occurring as arable weeds in Europe and native to or naturalised in other continents, was evaluated. In total, 3,712 and 802 seedlings representing 21 weed species were aphid and sap-inoculated with PVY, respectively. Experimentally-inoculated plants of Erodium cicutarium, Geranium pusillum, Lactuca serriola and Lamium purpureum tested positive by ELISA, but they did not display any disease symptoms. The presence of PVY in selected plants of the four species was confirmed by back-inoculations to Nicotiana tabacum and by immunocapture-reverse transcription-PCR performed on samples taken from weed and/or tobacco plants. Natural PVY infection in plants of the four wild species collected in the potatogrowing area was detected by ELISA and confirmed by back-inoculations to tobacco plants. This study is believed to be the first report of PVY infection in E. cicutarium, G. pusillum and L. purpureum. Moreover, our findings reveal for the first time PVY infection in L. serriola in central Europe.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.