Objective: Structural elucidation studies on Trichosanthes lobata ethyl acetate and methanol extracts of leaf and stem parts through Gas Chromatography-Mass Spectrometry (GC-MS) technique with respect to anti-inflammatory potential.
Methods: Extracts obtained with shade dried and powdered samples in successive solvent extraction using ethyl acetate and methanol by Soxhlet apparatus and subjected to GC-MS analysis and interpreted for its anti-inflammatory compounds.
Results: The study revealed that the extraction solvent used was able to recover compound of classes such as organic acid esters and conjugated alkaloids in larger quantities than other classes of compounds and they varied with leaf and stem and also with the polarity of solvents used. In total compounds identified, GC-MS profile of the Ethyl Acetate leaf extract of T. lobata contained 41 compounds, stem extract contained 45 compounds which have reported bioassays in PubChem. Whereas GC-MS profile of methanol leaf extract of T. lobata contained 66 compounds and stem extract contained 46 compounds having bioassay reports in PubChem. A large number of phytochemical peaks with good area percentage were found in methanolic extract. We were also able to find out potent anti-inflammatory compounds including Octanoic acid, Dodecanoic acid, Octadecane, Enoic acid, Hexanoic acid, Quinazolin-8-one, Ilicic acid, Pentadecanoic acid, Oxaspiro, Benzeneacetic acid, etc. from the extracts.
Conclusion: T. lobata contains phytocompounds against inflammation which may serve as a new drug lead of natural products origin in future and make it employable in modern pharmacological practices.
Introduction and Aim: Trichosanthes lobata is one of the species which belongs to Chinese traditional medicine for the therapeutic purpose of antioxidant properties. Free radicals’ production by the body has numerous beneficial roles including in immune systems, cellular signaling pathways, mitogenic response, and synthesis of cellular structures. This study aimed to evaluate the therapeutic efficacy of the T. lobata secondary metabolites toward COX proteins and validate their antioxidant potential.
Materials and Methods: We extracted the plant using Soxhlet and subjected it to various assays like DPPH and TEAC, followed by in silico analysis. The molecular docking and dynamic simulation have been analyzed with respect to the protein of interest against selected phytochemicals from T. lobata.
Results: We observed the significant outcome from DPPH and TEAC assays like reducing the capability in contrast to T. lobata followed by docking and dynamic stability.
Conclusion: The current findings have unveiled that the investigated flora, namely T. lobata, is a bastion of secondary phytochemicals. The plant's exceptional antioxidant capacity is attributable to the occurrence of one or more of these secondary metabolites, which exert their respective or synergistic effects.
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