Aim: To evaluate validity of the Greek version of a global measure of perceived stress PSS−14 (Perceived Stress Scale-14 item). Materials and Methods: The original PSS−14 (theoretical range 0−56) was translated into Greek and then back-translated. One hundred men and women (39±10 years old, 40 men) participated in the validation process. Firstly, participants completed the Greek PSS−14 and, then they were interviewed by a psychologist specializing in stress management. Cronbach's alpha (α) evaluated internal consistency of the measurement, whereas Kendall's tau-b and Bland & Altman methods assessed consistency with the clinical evaluation. Exploratory and Confirmatory Factor analyses were conducted to reveal hidden factors within the data and to confirm the two-dimensional character of the scale. Results: Mean (SD) PSS−14 score was 25(7.9). Strong internal consistency (Cronbach's α = 0.847) as well as moderate-to-good concordance between clinical assessment and PSS−14 (Kendall's tau-b = 0.43, p<0.01) were observed. Two factors were extracted. Factor one explained 34.7% of variability and was heavily laden by positive items, and factor two that explained 10.6% of the variability by negative items. Confirmatory factor analysis revealed that the model with 2 factors had chi-square equal to 241.23 (p<0.001), absolute fix indexes were good (i.e. GFI=0.733, AGFI=0.529), and incremental fix indexes were also adequate (i.e. NFI=0.89 and CFI=0.92). Conclusion: The developed Greek version of PSS−14 seems to be a valid instrument for the assessment of perceived stress in the Greek adult population living in urban areas; a finding that supports its local use in research settings as an evaluation tool measuring perceived stress, mainly as a risk factor but without diagnostic properties.