FIG 2. Basophils from allergic patients do not respond to TSLP and do not express IL-7Ra. Whole blood samples from HDs (empty bars, n 5 5) and allergic patients (black bars, n 5 8) were stimulated for 6 hours with IL-3, TSLP, anti-Fc 3RI antibody, or medium and analyzed by FCM. Gating strategy on whole blood (A). Intracellular P-STAT5 expression in basophils (left) and mDCs (right) (B). CD203c (left) and CCR3 (right) expression on basophils (C). % of IL-7Ra-and TSLPR-expressing basophils (left) or mDCs (right) (D). Data are shown as mean 6 SEM.