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The effect of palm oil mill effluent vermicompost on growth and vegetative traits of oil palm seedlings was evaluated. Different rates of vermicompost 10 g, 20 g and 30 g and 20 g of an NPK blue fertilizer as designated control was used. The experiment was conducted over a period of 120 days. Growth and vegetative traits like plant height, girth size, total dry weight (TDW) and root: shoot ratio (RSR) and foliar nutrient data were collected. Noticeably, the vermicompost and the NPK blue fertilizer were at par in stimulating the growth of the oil as shown in the plant height and girth size. The total dry weight (TDW) and Root: shoot ratio (RSR) showed that the vermicompost especially the higher rates significantly performed equal and positively affected the plant biomass. The nitrogen content was apparent in the plants grown with the highest rate of the vermicompost 30 g and the fertilizer with a significant effect on the leaf chlorophyll content. The results showed that the vermicompost particularly the higher rates were as suitable as the fertilizer with respect to the growth and vegetative traits.

Increasing trend in antimicrobial resistance and failure of chemically synthesized antibiotics lead to discover alternative methods for the treatment of bacterial infections. Various medicinal plants are in use traditionally and their active compounds can be further applied for treatment of bacterial diseases. This study was designed to determine the antibacterial activity of Punica granatum (P. granatum L.) (pomegranate) peel extract against Enterobacteriaceae [Escherichia coli (E. coli), Salmonella Typhimurium (S. Typhimurium) and Shigella Dysenteriae (S. Dysenteriae)] and gram-positive bacterium [Staphylococcus aureus (Staph aureus)]. Methanolic extract of P. granatum L. peel was prepared by Soxhlet apparatus method. Total flavonoid and phenolic contents from the extract were determined by High Performance Liquid Chromatography (HPLC). The antibacterial activity of P. granatum L. peel extract was evaluated through agar well diffusion method. HPLC showed the range of phenolics (gallic acid, caffeic acid, benzoic acid, cinnamic acid) and flavonoid compounds. The chemical structures of flavonoid and phenolics found in the methanolic extract of P. granatum L. peel have been reported for the first time. The methanolic peel extract (50 ul) of yellow P. granatum L. showed 26, 10, 10 and 9mm zones of inhibition (ZOI) against S. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. The methanolic extract of red P. granatum L. (100 ul) showed 27, 8, 12 and 15 mm ZOI against Staph. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. Highest ZOI was observed against Staph. aureus. Many of the bacteria studied in the present work may cause serious gastrointestinal infections, which can lead to hemorrhagic diarrhea in children. These infections can be life-threatening to young children and the elderly. There is an incentive to find alternative control measures, such as plant and herbal extracts, especially in lesser-developed countries where traditional antibiotics may not be readily available.

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In Pakistan, salinization of agricultural lands is a serious problem prevailing in arid and semi-arid regions of the country. The main objective of the present study was to investigate the carbohydrates dynamics of the salt-affected soil, amended with different organic matter combinations and incubated for varying time periods (0, 15, 30 and 60 days) and temperatures (15 and 37 o C). Replenishment of soil organic matter (SOM) was made with Green manure (GM) and Farmyard manure (FYM). The soil pH, EC, Na + and K + contents were evaluated through standard methods. An improvement in the soil organic carbon (OC) and nitrogen (N) contents ranging from 0.10 to 1.40% and 0.05 to 0.55% was achieved through the selected soil amendment practices. Total carbohydrates were found to be enhanced with organic treatments alone or in combination, accounting for 13.20% to 26.30% of the total soil. Non-glycosidic interactions of soil carbohydrates with minerals were investigated by applying powdered X-Ray diffraction (XRD) analysis using a Rigaku wide range goniometer (PW 1050/23). Semi-quantitative analysis of the soil organomineral interactions, based on their relative frequency abundance, was expressed in terms of a system of nine components. X-Ray diffraction (XRD) analysis revealed interactions of varying magnitude namely major, minor, trace and rare between the soil carbohydrates and minerals.

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In present study, the effect of different solvents and multiple techniques on the extraction yield, phenolic contents and antioxidant activities of bark of F. Religiosa, was investigated. Four solvents (absolute ethanol, absolute methanol, 80% aqueous ethanol and 80% aqueous methanol) and three techniques (orbital shaker, sonication and magnetic stirrer) were applied for this purpose. The extract obtained by the application of 80% methanol, exhibited significantly (p<0.05) higher antioxidant activities. The statistical order of the solvents according to their efficiency was 80% methanol > 80% ethanol > absolute methanol >absolute ethanol. As for as techniques are concerned, the extracts obtained by the application of sonication demonstrated significantly (p<0.05) higher antioxidant activities as compared to to the extracts obtained as a result of using magnetic stirrer and orbital shaker.