The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation of the RNAi-based gene construct targeting V2 gene of CLCuKoV-Bur, cloned under 35S promoter, was done in two elite cotton varieties MNH-786 and VH-289 using shoot apex cut method of gene transformation. The transformation efficiency was found to be 3.75 and 2.88 % in MNH-786 and VH-289, respectively. Confirmation of successful transformation was done through PCR in T 0, T 1, and T 2 generations using gene-specific primers. Transgenic cotton plants were categorized on the basis of the virus disease index in T 1 generation. Copy number and transgene location were observed using FISH and karyotyping in T 2 generation which confirmed random integration of V2 RNAi amplicon at chromosome 6 and 16. Real-time quantitative PCR analyses of promising transgenic lines showed low virus titer compared to wild-type control plants upon challenging them with viruliferous whiteflies in a contained environment. From the results, it was concluded that amplicon V2 RNAi construct was able to limit virus replication and can be used to control CLCuV in the field.
Transparency order is considered to be a cryptographically significant property that characterizes the resistance of S-boxes in opposition to differential power analysis attacks. The S-box having low transparency order is more resistant to these attacks. Until now, little attempts have been noticed to examine theoretically the transparency order and its relationship with other cryptographic properties. All constructions associated with transparency order are relying on search algorithms. In this paper, we discuss the new interpretation of bent functions in terms of their transparency order. Using the concept of vector concatenation and correlation characteristics, we find the transparency order of Boolean functions. The notion of complementary transparency order is given. For a pair of Boolean functions, we interpret complementary transparency order by their Walsh-Hadamard transform. We establish a relationship of transparency order with cross-correlation for a pair of Boolean functions. We find a relationship of transparency order with (n − 2)−variable decomposition bent functions. We generalize the bounds on sum-of-squares of autocorrelation in terms of transparency order of Boolean functions using Walsh-Hadamard spectra. Further the transparency order of a function fulfilling the propagation criterion about a linear subspace is evaluated.
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