A recombinant plasmid vector was constructed in which the bacterial LacZ gene was placed under the control of a Bombyx mori baculovirus early promoter. The vector proved to be active in transfected cultured dipteran and lepidopteran cells. Co-transfection carried out with this recombinant plasmid vector and a plasmid containing the hygromycin phosphotransferase gene followed by selection with the antibiotic hygromycin B, resulted in stable transformation of cultured Drosophila melanogaster Schneider 2 cells. Southern blot analysis of the host cell's genomic DNA in combination with chromosomal in situ hybridization demonstrated that multiple copies of both plasmids were integrated in the host cell's genome.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.