Electronically excited triplet carbonyls are formed during the oxidative degradation of polyunsaturated fatty acids, amino acids, and beta-dicarbonyl metabolites. Due to their long lifetime and high alkoxyl radical-like reactivity, triplet carbonyls may initiate deleterious reactions in biological systems. Here we study the quenching properties of conjugated dienes, specifically 2,4-hexadienoate (sorbate) and its alkyl ester, on triplet acetone generated chemically (thermolysis of tetramethyl-1,2-dioxetane) or enzymatically (horseradish peroxidase-catalyzed aerobic oxidation of isobutanal). Triplet acetone quenching rates were near diffusion control ( k q = 10 (8)-10 (9) M (-1) s (-1)) and accompanied by diene cis-trans isomerization. None of the dienes displays antioxidant activity in classical systems known to generate reactive oxygen species: superoxide anion radical, hydroxyl radical, alkoxyl and alkylperoxyl radicals, or singlet oxygen. Experiments with model systems used widely to study lipid peroxidation showed that sorbate can inhibit mitochondrial swelling induced by enzymically formed triplet benzophenone and quench the chemiluminescence of microsome preparations challenged with iron and ascorbate. Altogether, our data indicate that conjugated dienes can be used as specific quenchers of triplet carbonyls formed in biological systems during oxidative stress. Moreover, they suggest that the well-known food preservative properties of sorbate may be due to its triplet carbonyl quenching activity.
Parameters that influence the zero valent iron mediated degradation of the pharmaceutical diazepam (DZP) were evaluated including the iron concentration and its pre-treatment, the effect of complexation with EDTA and oxic versus anoxic condition. It was observed that acid pre-treatment of iron particles is important for degradation efficiency and that H(2)SO(4) is a better choice than HCl, resulting in higher degradation of DZP. Under oxic conditions, the degradation of DZP achieved 96% after 60 min using Fe(0) (25 g L(-1)) pre-treated with H(2)SO(4) in the presence of EDTA (119 mg L(-1)), while mineralization achieved around 60% after the same time. Under anoxic conditions, degradation occurred, however at lower extent, achieving 67% after 120 min. The addition of EDTA improved the treatment efficiency in 20% leading to 99% DZP degradation after 120 min. The first intermediates formed during DZP degradation were identified using LC/MS analysis and revealed the formation of mono- and di-hydroxylated products from DZP during Fe(0)/EDTA/O(2) degradation, which evidences that (·)OH was the main oxidizing species formed in this process.
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