BackgroundThis study describes the ultrasonographic findings of the rumen in 45 healthy dairy cows.ResultsThe cows were scanned on both sides using a 5.0 MHz transducer. The dorsal visible margin of the rumen ran parallel to the lung from cranioventral to caudodorsal. It was furthest from the dorsal midline at the 9th intercostal space (48.3 ± 9.24 cm) and closest at the 12th intercostal space (22.4 ± 3.27 cm). The longitudinal groove, which could be clearly identified at all examination sites because it appeared as a triangular notch, formed the ventral margin of the dorsal sac of the rumen. The dorsal sac of the rumen was largest at the caudal flank (40.3 ± 6.33 cm), where it was adjacent to the abdominal wall. The ventral sac of the rumen extended across the ventral midline into the right hemiabdomen and its ventral margin had a largely horizontal craniocaudal course. The height of the ventral sac of the rumen exceeded that of the dorsal sac at all examination sites; the maximum height was measured at the 12th intercostal space (62.6 ± 9.53 cm). The dorsal gas cap, characterised ultrasonographically by typical reverberation artifacts, was visible in all cows from the 12th intercostal space to the caudal flank. It was largest at the 12th intercostal space (20.5 ± 7.03 cm). The transition from the gas cap to the fibre mat was marked by the abrupt cessation of the reverberation artifacts. It was not possible to differentiate a fibre mat and a ventral fluid phase. The rumen could be imaged from the right side in 21 cows (47%).ConclusionsUltrasonography is well suited for the detailed examination of the rumen of cows. The reference values obtained from this study add to the diagnostic tools that are available for the assessment of bovine patients.

The goal of this study was to investigate the feasibility of ultrasonographic assessment of reticuloruminal motility in 45 healthy cows. The transducers of five ultrasound machines were connected to a digital video recorder and placed simultaneously at five sites on the left side of the cows to scan the reticulorumen (reticulum; ruminal atrium; dorsal sac of the rumen; left longitudinal groove; ruminal recess, caudodorsal and caudoventral blind sacs). The video streams from all five ultrasound machines were recorded synchronously with the same time line and displayed on a single monitor. Time 0 was defined as the start of a biphasic reticular contraction. The reticulum was visualised in all cows and had 11.0 ± 2.12 biphasic contractions in 9 min. The ruminal atrium was visualised in 40 (89%) cows and had 10.7 ± 2.10 contractions in 9 min, which started at the time point 5.0 ± 0.83 sec and lasted 7.0 ± 2.14 sec. Contractions of the dorsal sac of the rumen, visible in all cows, were visualised in 29 (64%) cows. There were 9.5 ± 1.8 contractions in 9 min that started at the time point 4.0 ± 0.85 sec and lasted 8.2 ± 1.04 sec. The left longitudinal groove was seen contracting in 39 (87%) cows. There were 10.2 ± 1.98 contractions in 9 min that started at the time point 4.1 ± 1.81 sec and lasted 7.8 ± 1.19 sec. Contractions of the ventral sac of the rumen (ruminal recess) were seen in 31 (69%) cows. There were 7.5 ± 2.59 contractions in 9 min that started at the time point 14.3 ± 4.30 sec. Contractions of the caudodorsal and caudoventral blind sacs were seen in 34 (76%) cows. There were 9.0 ± 2.75 (1.0 ± 0.31) contractions/min and 9.4 ± 2.09 (1.0 ± 0.23) contractions/min of the dorsal and ventral blind sacs, and they started at the time points 6.2 ± 1.32 sec and 21.3 ± 6.20 sec, respectively. Primary contraction cycles were seen in all cows and secondary cycles in 22 (49%) cows. The former were complete in 37 (82%) cows and incomplete in 8 (18%). There were 11.0 ± 2.12 primary and 4.5 ± 2.15 secondary cycles in 9 min, and the ratio between primary and motilisecondary cycles averaged 2.4:1. Ultrasonography is suitable for the assessment of reticuloruminal motility in cattle. cows. There were 10.2 ± 1.98 contractions in 9 min that started at the time point 4.1 ± 1.81 sec and lasted 7.8 ± 1.19 sec. Contractions of the ventral sac of the rumen (ruminal recess) were seen in 31 (69 %) cows. There were 7.5 ± 2.59 contractions in 9 min that started at the time point 14.3 ± 4.30 sec. Contractions of the caudodorsal and caudoventral blind sacs were seen in 34 (76 %) cows. There were 9.0 ± 2.75 (1.0 ± 0.31) contractions/min and 9.4 ± 2.09 (1.0 ± 0.23) contractions/min of the dorsal and ventral blind sacs, and they started at the time points 6.2 ± 1.32 sec and 21.3 ± 6.20 sec, respectively. Primary contraction cycles were seen in all cows and secondary cycles in 22 (49 %) cows. The former were complete in 37 (82 %) cows and incomplete in 8 (18 %). There were 11.0 ± 2.12 primary and 4.5 ± 2.15 secondary cycles in 9 min, ...

In der Milchviehhaltung sind antibiotische Tierarzneimittel, welche ihre Wirkung auf Mensch, Tier und Umwelt ausüben, ein öffentlich kontrovers diskutiertes Thema. In dieser Studie soll aufgezeigt werden, wie Betriebe bestehen, die keine antibiotischen Euterpräparate einsetzen. Dabei soll es nicht zu einer Vernachlässigung des Tierwohls und der angestrebt tiefen Tankmilchzellzahlen kommen. Die Betriebsdaten wurden während der Fütterungszeit und einem Melkvorgang auf 18 Schweizer Betrieben, welche seit mindestens 3 Jahren im Euterbereich keine Antibiotika einsetzten, erhoben und in dieser Studie ausgewertet. Dabei handelt es sich um eine Querschnittsstudie, welche Prävalenzen präsentiert. Die Landwirte setzten auf Zuchtmerkmale wie eine hohe Lebensleistung, eine tiefe Milchzellzahl über mehrere Laktationen hinweg, einen friedlichen Tiercharakter und auf kleinrahmige Kühe mit tiefem Flankenbau. Es wurde ein Trend Richtung Zweinutzungsrassen beobachtet. Die Bauern achteten darauf, dass nur wenige verschiedene Personen regelmässig melkten. Aufgrund der Aufzucht eigener Jungrinder auf 94% der Betriebe, wurde der Tierverkehr reduziert. Weiter hatten klare Betriebsstrukturen und Arbeitsabläufe, sowie eine gute Tierbeobachtung einen entscheidenden Einfluss auf den Erfolg dieser Produktionsweise. Unterstützend kamen auf diesen Betrieben eigene Therapieformen, wie Homöopathie, Phytotherapie und altbewährte Hausmittel zum Einsatz.

Background:Complex regional pain syndrome (CRPS) is a rare painful condition that usually appears after trauma or surgery of the extremities. Symptoms include pain, sensory, sudomotor and vasomotor disturbances, trophic changes and impaired motor function. The course varies from mild to chronic disease with a high impact on daily functioning and quality of life. In skin tissue, sustained inflammatory, fibrotic processes together with reduced epidermal nerve fibers are reported. Neurotrophins and their receptors are mediators in cell-to-cell communication and key mediators of pain signalingObjectives:The aim of this study was to identify differential expression of neurotrophins and their receptors in the skin and skin fibroblasts of patients with CRPSMethods:Healthy controls (HC) and patients with acute CRPS with symptoms for less than 6 months fulfilling the Budapest criteria were recruited. Pain scores were evaluated by numeric rating scale (0=no pain, 100=maximal) and body perception was assessed using the Bath Body Perception Disturbance Scale (BBPDS) (0=no perception disturbance, 57=maximal perception disturbance).Skin biopsies of the affected and/or non-affected side were taken. Immunohistochemistry on formalin-fixed, paraffin-embedded skin tissue slides was used to show NT3 expression in skin tissues. Blinded analysis was done by an experienced dermato-pathologist determined staining graduated by 0= none, 1= sparse, 2=moderate, 3= dense.Skin fibroblast were isolated from skin biopsies by outgrowth cultures (CRPS, affected side, n=6 and HC, n=5). Cells (passage 3-6) were starved and subsequently stimulated with TNFα (10 ng/ml) or TGFβ (10 ng/ml) for 24 h to mimic active disease and total RNA was isolated by miRNeasy kit. Gene expression of neurotrophins (NGF, BDNF, and NT3) and neurotrophin receptors (NGFR, TrkA, TrkB and TrkC) was measured by quantitative real time PCR and quantified using the ΔΔCq method with GAPDH as a reference gene. ELISA was used to analyze NT3 protein expression in cell culture supernatants.Results:In 5 of 9 patients with CRPS immunohistological staining of NT3 showed an higher expression (from low to moderate) in the affected side versus the non-affected side. In 4 of 9 patients the expression of NT3 was high in the non-affected side (moderate/dense) and stayed high in the affected side.Of interest, the patients with increasing expression of NT3 in the affected side showed increased pain scores (max pain 80+/-10.95, n=5 versus 48.16+/-18.16, n=4, p=0.059 and changed body perception 26.8+/-8.68 n=5 versus 6.5+/-3.91, n=4, p=0.016).Isolated skin fibroblasts from the affected side of patients with CRPS compared to healthy skin fibroblasts showed higher basal gene expression of NT3 (log2 fold-change= 1.9 +/- 0.4, p= 0.005) and NGFR (log2 fold-change= 3.6 +/- 2.1, p=0.014). TNFα stimulated CRPS skin fibroblasts showed higher expression for NT3 (log2 fold-change= 2.1 +/- 1.2, p=0.002) compared to HC. TGFb stimulated skin fibroblasts of patients with CRPS showed higher expression of NT3 (log2 fold-change= 1.4+/-0.8, p=0.019), NGFR (log2 fold-change= 2.6 +/- 1.8, p=0.036) and TrkC (log2 fold-change= 2.3 +/- 1.8, p=0.032) compared to HC.On protein level, NT3 showed a tendency of upregulation in unstimulated fibroblasts from CRPS patients comparing to HC (CRPS mean= 8.0 +/- 2.2 pg/ml, HC mean= 6.3 +/- 1.8 pg/ml, p=0.25). After TNFα stimulation, protein level of NT3 was significantly higher in CRPS skin fibroblasts (CRPS mean= 10.6 +/- 2.4 pg/ml, HC mean= 4.8 +/- 1.3 pg/ml, p=0.004).Conclusion:These data indicate a new role of skin fibroblasts in CRPS. Differential basal and stimulated expression of NT3, the receptor for NT3 (TrkC) and NGFR, the common receptor for all neurotrophins, indicates deregulated communication of fibroblasts with the sensory nerve fibers in CRPS. This might contribute to the dysregulated healing process and sustained pain.Disclosure of Interests:Sanne Stroeve: None declared, Stefan Dudli: None declared, Isabel Kolm: None declared, Irina Heggli: None declared, Nick Herger: None declared, Sabrina Catanzaro: None declared, Andreas Schweizer: None declared, Maurizio Calcagni Speakers bureau: Arthrex, Consultant of: Medartis, Arthrex, SilkBiomaterials, Grant/research support from: Medartis, Oliver Distler: None declared, Florian Brunner: None declared, Astrid Juengel: None declared