The chemical constituents and biological activity of Pinus cembra L. (Pinaceae), native to the Central European Alps and the Carpathian Mountains, are not well known. The aim of the present work was to examine the phenolic content, antioxidant and antimicrobial effects of hydromethanolic extracts of Pinus cembra L. bark and needles. Bark extract had higher concentrations of total phenolics (299.3 vs. 78.22 mg gallic acid equivalents/g extract), flavonoids (125.3 vs. 19.84 mg catechin equivalents/g extract) and proanthocyanidins (74.3 vs. 12.7 mg cyanidin equivalents/g extract) than needle extract and was more active as a free radical scavenger, reducing agent and antimicrobial agent. The EC50 values in the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing power assays were 71.1, 6.3 and 26 μg/mL for bark extract and 186.1, 24 and 104 μg/mL for needle extract, respectively. In addition, needle extract showed ferrous ions chelating effects (EC50 = 1,755 μg/mL). The antimicrobial effects against Staphylococcus aureus, Sarcina lutea, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans were assessed by the agar diffusion method. Both extracts (4 mg/well) were active against all the microorganisms tested; bark extract showed higher inhibition on all strains. These results indicate that Pinus cembra L. bark and needles are good sources of phytochemicals with antioxidant and antimicrobial activities.

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There is great evidence regarding the beneficial influence of rose hips on human and animal health. In this respect, fruits of native populations of Rosa spp. collected from the northeastern part of Romania (400-1060 m altitude) were assayed for nutritional and some morphological properties, in order to select the best genotypes. The results show a great quantitative variability of analyzed genotypes, regarding dimensions and biochemical indicators as follow: ascorbic acid: 866.91 mg/100 g fw (R. rubiginosa), 615.98 mg/100 g fw (R. caesia); carotenes: 34.95 mg/100 g fw (R. subcanina), 24.64 mg/100 g fw (R. nitidula); protein content: 0.47 g/100 g fw (R. micrantha), 0.25 g/100 g fw (R. caesia); total sugars: 17.63 g/100 g fw (R. andegavensis), 11.55 g/100 g fw (R. caryophyllacea). The fruits length, width and dry weight were: 25 mm (R. canina), 12 mm (R. micrantha), 16.5 mm (R. corymbifera), 8 mm (R. micrantha) and 25% dry weight (R. rubiginosa), 38% dry weight (R. nitidula), respectively. The present study demonstrates the existence of a rich rose germplasm source in north-east area, with good biotechnological fruit quality, which recommends them as functional foods according to their chemical contents (R. rubiginosa, R. vosagiaca, R. subcanina, R. canina and R. caryophyllacea) and also as sources for natural colorants in food technology.

Phenotypic plasticity can mask population genetic differentiation, reducing the predictability of trait-environment relationships. In short-lived plants, reproductive traits may be more genetically determined due to their direct impact on fitness, whereas vegetative traits may show higher plasticity to buffer short-term perturbations. Combining a multi-treatment greenhouse experiment with global field observations for the short-lived Plantago lanceolata, we 1) disentangled the genetic and plastic responses of functional traits to a set of environmental drivers and 2) assessed the utility of trait-environment relationshisps inferred from observational data for predicting genetic differentiation. Reproductive traits showed distinct genetic differentiation that was highly predictable from observational data, but only when correcting traits for differences in their (labile) biomass component. Vegetative traits showed higher plasticity and contrasting genetic and plastic responses, leading to unpredictable trait patterns. Our study suggests that genetic differentiation may be inferred from observational data only for the traits most closely related with fitness.

Abstract:The paper presents the results of the studies performed in the Natura 2000 site Pădurea Bârnova-Repedea (ROSCI0135), in order to identify and map habitat types and plant species of community interest. Palynological and archaeo-botanical studies in the region were reviewed, in order to assess the presence of the ancient forests. This protected area was designed to preserve forests from two habitats types of community interest, namely Asperulo-Fagetum beech forests and Dacian oak-hornbeam forests, and to shelter a species of wild and endangered orchid, Cypripedium calceolus L. During the field work other Natura 2000 habitats of community interest have been identified: mountain hay meadows, Ponto-Sarmatic steppes and Ponto-Sarmatic deciduous thickets. The information obtained provided new data on the habitats and plant species distribution. Field data were correlated with existing topographic maps and aerial photography. The use of the Geographic Information System allowed translating the field distribution of the species and habitat types into accurate maps, which can be used in the future by the managers of this site for implementing adaptive conservation measures. The human activities with potential impact on this Natura 2000 site have been recorded. The map of current pressures on the site was generated based on these impact categories.