Our food security depends on finding a sustainable alternative to rock phosphate for fertilizer production. Furthermore, over 2 billion people worldwide are currently affected by micronutrient deficiencies, and crop concentrations of essential minerals are declining. This paper examines whether a novel multi-element fertilizer, Thallo ® , can produce crop yields comparable to conventional rock phosphate derived fertilizers, and have an additional benefit of increasing essential mineral concentrations. Thallo ® , produced from abattoir and recycled industrial by-products, was tested against conventional mineral fertilizers in a pot trial with wheat and grass. In soil, yields were comparable between the fertilizer types, but, in a low-nutrient substrate, Thallo ® showed a yield benefit. Elemental concentrations in the plant material typically reflected the relative concentrations in the fertilizer, and Thallo ® fertilized plants contained significantly more of some essential elements, such as selenium and zinc. Furthermore, concentrations of the toxic element cadmium were significantly lower in Thallo ® fertilized crops. Among the fertilizers, manganese concentrations were greatest in the Thallo ® , but within the fertilized plants, they were greatest under the mineral fertilizer, showing the complexity of assessing whether nutrients will be taken up by crops. In summary, fertilizers from livestock waste have the potential to improve wheat and grass concentrations of essential elements while maintaining yields.
Semi-volatile residues on aerospace hardware can be analyzed using Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFTS). This method can be correlated with quantitative Mil-STD 1246 NVR measurements while simultaneously providing qualitative identification of a large variety of compounds. Its high sensitivity supports the direct sampling of small areas of critical surfaces. This method involves transferring the contaminant film to a small solvent-saturated wipe, followed by extraction of the wipe, then concentration of the solvent extract and subsequent spectroscopic analysis using an FT-IR with a diffuse reflectance accessory. A library of standard curves for different classes of typical aerospace contaminants has been established. Quantitative analysis has been proven successful over orders of magnitude and detection limits exceeding 0.1 ug/cm 2 are routinely achieved. Several practical applications have been performed using this analytical method and detailed discussion of analysis techniques is presented. The discussion will include: instrumentation setup, selection and preparation of sample collection materials, sample extract preparation, preparation of standard calibration curves and spectral interpretation.
Posttranscriptional tRNA modifications are essential for proper gene expression, and defects in the enzymes that perform tRNA modifications are associated with numerous human disorders. Throughout eukaryotes, 2′- O -methylation of residues 32 and 34 of the anticodon loop of tRNA is important for proper translation, and in humans, a lack of these modifications results in non-syndromic X-linked intellectual disability. In yeast, the methyltransferase Trm7 forms a complex with Trm732 to 2′- O -methylate tRNA residue 32 and with Trm734 to 2′- O -methylate tRNA residue 34. Trm732 and Trm734 are required for the methylation activity of Trm7, but the role of these auxiliary proteins is not clear. Additionally, Trm732 and Trm734 homologs are implicated in biological processes not directly related to translation, suggesting that these proteins may have additional cellular functions. To identify critical amino acids in Trm732, we generated variants and tested their ability to function in yeast cells. We identified a conserved RRSAGLP motif in the conserved DUF2428 domain of Trm732 that is required for tRNA modification activity by both yeast Trm732 and its human homolog, THADA. The identification of Trm732 variants that lack tRNA modification activity will help to determine if other biological functions ascribed to Trm732 and THADA are directly due to tRNA modification or to secondary effects due to other functions of these proteins.
Transforming growth factor β (TGFβ) signals through the TGFβ Receptor 1 (TGFβR1) and is implicated in many aspects of malignancy. TGFβR1 gene is frequently mutated in ovarian carcinomas (OvCa) (Chen, T., et al, Cancer Research 61, 4679-4682, 2001). Susceptibility to numerous cancers is linked to two germline variants of TGFβR1, a G to A single nucleotide polymorphism in intron 7 (Int 7G24A) and a nine base pair deletion in exon 1 (TGFβR1*6A) although the mechanism(s) for this association is still unclear. Since the canonical pathway for TGFβR1 signaling is via phosphorylation of SMAD, the goal was to determine the association of either or both variants with development of subtypes of OvCa and to measure phosphorylation of pSMAD in the epithelium and stroma of OvCas from women with either or both Int 7G24A and TGFβR1*6A. FFPE tissues from 122 women without a history of cancer, and 59 women with OvCa were obtained from St. Elizabeth Healthcare (N. KY) and from 63 women with OvCa through the Cooperative Human Tissue Network (Birmingham Ala). OvCa patients and non-cancer controls were age matched. St. Elizabeth Healthcare IRB gave permission for this study. Tumors were diagnosed in H&E stained histologic sections by a Board Certified Pathologist (LED) and were classified as either Low Malignant Potential (LMP), Type 1 (clear cell, mucinous and low grade serous and endometrioid) or Type 2 (high grade serous, high grade endometrioid, or carcinosarcoma). Variants were identified in extracted DNA from FFPE tissues using PCR, capillary electrophoresis (CE), RFLP, and SSCP. Histologic sections were stained by IHC using the DAKO LSAB2 kit (Agilent) and anti pSMAD 2 (Millipore AB3849-1). Stain was evaluated as cytoplasmic and nuclear Histoscores (stain Area X Intensity) by two observers (LED, JHC). Data were evaluated by “Gene Code” with Gene 1 wild type for both variants, Gene 2 homozygous or heterozygous for Int 7G24A, Gene 3 homozygous or heterozygous for TGFβR1*6A, and Gene 4 having both variants. Usual chi-tests were used to determine significance of contingency tables, ANOVA with multiple comparison adjusted were used for markers. KM plots and proportional hazards were used in survival tests. The frequency in Gene 1, 2, 3, and 4 was significantly different in controls vs. OvCa patients (p = 0.0010); 57.7% of OvCa patients had a TGFβR1 variant vs. 36.6% of controls. Frequency of TGFβR1 variants in 62 patients with Type 2 OvCa was 62.9%. Patient survival differed significantly between patients with LMP, Type 1, and Type 2 OvCa (p <0.0001). Differences in nuclear expression of pSMAD in both tumor epithelial and stromal cells between OvCa types were highly significant (p < 0.0001, p< 0.0092). pSMAD expression decreased significantly in the nuclei of both epithelial and stromal cells in Type 2 tumors. These data indicate that germline variations in the TGFβR1 gene are associated with high grade ovarian cancers and altered SMAD phosphorylation. Citation Format: Julia H. Carter, James P. Schaeper, Taiping Chen, Diane W. Fritz, Leah Focke, Adrian Guy, James A. Deddens, Larry E. Douglass. Alterations in TGFβ signaling in ovarian cancer patients with TGFβ receptor 1 variants [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2267.
Transfer RNAs undergo post‐transcriptional modifications, including many which occur at or near the anticodon loop and are necessary for protein synthesis. In Saccharomyces cerevisiae, Trm7 is required for 2’‐O‐methylation of tRNA and requires interaction with Trm732 and Trm734 for modifications at positions 32 and 34, respectively. These proteins are widely conserved in eukaryotes, and mutations in the human ortholog of TRM7, FTSJ1, can cause non‐syndromic X‐linked intellectual disability by decreasing or eliminating methylation activity. Through a genetic assay, we have found Trm7 residues required for interaction with Trm732 or Trm734 and the subsequent methylation of tRNAPhe. These spot test assays showed that the Trm7 Y138R variant abrogates interaction with Trm734 and the Trm7 I144A variant abrogates interaction with Trm732. To verify that these genetic results are due to impaired interaction of Trm7 variants with its binding partners, we are performing immunoprecipitation assays. In addition, we have begun to study interactions of the individual proteins and complexes with tRNAPhe through biochemical methods such as electrophoretic mobility shift assays, to determine the role of each protein in tRNA binding. Through this study of Trm7 variants, we can better understand the interaction of Trm7 with Trm734 and Trm732, which will facilitate the study of conserved homologs in humans.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.