Our study of the diversity at various time points from the ETP provided an overview of the shifts of the catabolic composition of the sludge. This also depends on the influential parameters like the incoming pollutant level and the environmental conditions that are prevailing and often changing from time to time. The results of direct DNA extraction and PCR amplification do reflect the relative abundance of a particular catabolic genotype, which could be used to monitor the efficiency of treatment.
The requirements for treated wastewater are becoming increasingly more stringent, and therefore the improved efficiency of biological treatment processes is indispensable at industrial effluent treatment plants (ETPs). Microorganisms such as bacteria play an important role in the natural cycling of materials and particularly in the decomposition of organic wastes. The knowledge of the interactions among these microbial populations needs to be harnessed for optimum evaluation and functioning of effluent treatment plants. Modern molecular techniques have revolutionized the methods of assessing these microbial populations. The combination of the results of these microbial assessments along with the on-site parameters at ETPs would favor an efficient treatment. In this review, the various approaches and importance of correlating the microbial population dynamics and treatment of wastewater at industrial ETPs has been elaborated.
This study demonstrates the effect of various nitrogen levels in the medium along with different carbon sources in the media on the utilization of phenol by Pseudomonas CF600. Experiments were carried out using cultures derived from minimal media containing the carbon sources phenol and citrate, followed by varying nitrogen levels in the medium. Respirometric analysis under different conditions was carried out with cells using phenol and catechol as substrates. When nitrogen was limiting in the medium, phenol induced higher oxygen uptake rates, whereas catechol was independent of the nitrogen levels. These observations were also supported by the residual phenol levels in the medium with different levels of nitrogen as NH(4) ion. Results show that nitrogen-limiting conditions favor the phenol utilization by Pseudomonas CF600.
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