The aim of this study was to clone native Anatolian Grey cattle by using different donor cell types, such as fibroblast, cartilage and granulosa cells cryopreserved in a gene bank and oocytes aspirated from ovaries of Holstein cows as the recipient cytoplasm source. One male calf from fibroblast, three female calves from granulosa cells and one female calf from cartilage cells were born healthy and at normal birthweights. No calves were lost after birth. The results demonstrated that the cloned calves had the same microsatellite alleles at 11 loci as their nuclear donors. However, the mtDNAs of the five Anatolian Grey cloned calves had different haplotypes from their donor cells and mtDNA heteroplasmy could not be detected in any of the clones. The birth of healthy clones suggests that the haplotype difference between the cell and oocyte donor did not affect the pre- or post-implantation development of the bovine nuclear transfer derived embryos in our study. The results showed that well established nuclear transfer protocols could be useful in conserving endangered species. In conclusion, somatic cell banking can be suggested as a tool in conservation programmes of animal genetic resources.

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Makale Kodu (Article Code): KVFD-2009-1185 ÖzetBu çalışmada, iki haftadır devam eden vagina kökenli kanama şikayeti ile getirilen, kısırlaştırılmış, 2.5 yaşlı ve 26 kg vücut ağırlığına sahip melez dişi bir köpeğin klinik ve patolojik değerlendirilmesi yapıldı. Olgunun 6 aylıkken ovaryohisterektomi ameliyatı geçirmiş olmasına rağmen erkek köpeklere ilgi gösterdiği hatta çiftleştiği anamnez olarak alındı. Yapılan klinik muayenede, vagina kökenli kanama ve orificium urethralis externa'nın önünde, sol lateral duvarda yaklaşık 50 mm çapında, sert ve karnıbahar biçiminde bir oluşum tespit edildi. Total lökosit sayısında artış ve hiperglisemi saptandı. Vaginal sitolojide nötrofiller, eritrositler, bulaşıcı veneral tümör hücreleri, intermediyer ve parabazal hücreler tespit edildi. Abdominal ultrasonografi ile her iki böbreğin gerisindeki ovaryumlar ve bunlarla bağlantı halinde olan cornu uteriler ile uyumlu parçalar anekoik yapıda görüntülendi. Median hattan yapılan laparatomi ile ovaryum ve uterus olduğu düşünülen dokular uzaklaştırıldı. Bu dokuların histopatolojik yoklamalarında pyometra ve sol ovaryumda kist ve papiller hiperplazi teşhis edildi. Olguya 7 hafta boyunca, haftada bir kez vincristine, %0,9'luk sodyum klorür solüsyonu ile birlikte infüzyon şeklinde uygulandı. İlk uygulamanın ardından kanamanın durduğu ve lezyonun küçüldüğü saptandı. Ancak dördüncü haftadan son uygulamaya kadar lezyonun büyüklüğünde ve renginde bir değişikliğin olmadığı tespit edildi. Tedaviden 10 ay sonra yapılan kontrolde, kanamanın bulunmadığı görüldü ve kitle tespit edilemedi.Anahtar sözcükler: Köpek, TVT, Stump pyometra, Ovaryohisterektomi Concomitant Occurrence of Ovarian Remnant Syndrome, Transmissible Venereal Tumor and Stump Pyometra in A BitchSummary A 2.5-year-old, cross-breed bitch weighing 26 kg, was presented with two weeks history of vaginal bleeding. The bitch had undergone an ovariohysterectomy when it was 6 months old, and it had been regularly showing attractiveness to male dogs and even being mated with free-roaming dogs. On physical examination, the animal appeared healthy with no abnormalities other than vaginal bleeding. Upon vaginal digital palpation and vaginoscopic examination, an area of rough, cauliflower-like mucosa approximately 50 mm in diameter was determined on the left lateral wall just cranial to the urethral orifice. The dog had leukocytosis and hyperglycemia. Vaginal cytology revealed huge numbers of red blood cells, neutrophils, transmissible venereal tumor cells and some degree of intermediate and parabasal cells. Abdominal ultrasonography demonstrated two cystic structures with anechoic areas next to each kidney. The residual ovaries with cystic uterine remnants were removed via a midline laparotomy. Histopathological examination of the removed tissues revealed pyometra and cyst and papillary hyperplasia in the left ovary. Chemotherapy with slow infusions of vincristine was performed once a week for seven consecutive weeks. After the first vincristine administration, cessation of the vaginal bleeding...

SummaryVaginal leiomyosarcoma was clinically, histopathologically and immunohisto-chemically detected in a 4 year old Holstein cow in this case. The mass was extirpated and no recurrence was observed in the vagina at 12 months follow up. The cow was artificially inseminated and became pregnant after the surgery.

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