In our experience, only p16 and p21 may be useful in the management of superficial bladder tumors, as they are predictors of recurrence and survival in Ta and T1 patients.
RESUMEN VALIDACIÓN HISTOPATOLÓGICA DE LA TECNOLOGÍA TISSUE-MICROARRAY DE CÁNCER DE UROTELIO.NUESTRA EXPERIENCIA INTRODUCCIÓN: La tecnología array ofrece: gran ventaja a los investigadores clínicos y básicos, facilita aplicar gran cantidad de técnicas (inmunohistoquímica, FISH, proteómica) para comprender los mecanismos moleculares del cán-cer, ofrece economía de escala en los reactivos versus los procedimientos convencionales. Dado que la representación de la muestra es muy reducida, es exigible previamente validar el array. MATERIAL Y MÉTODOS:A partir de bloques de parafina de carcinomas de urotelio almacenados, cuya antigüedad oscilaba entre 5-7 años, se han seleccionado 52 casos consecutivos; se ha construido un array de tejido; los discos se colocaron en filas y columnas de manera aletoria, dibujando un topograma para guía de lectura. Se validó por otro pató-logo ajeno a la selección de las muestras.RESULTADOS: Se han obtenido 87 laminillas. La número 1 se ha teñido con HE. Ha habido discrepancia en el 27% de las muestras en el estadiaje. No ha existido discrepancia en el diagnóstico histológico. En 11 puntos (17%) no hay representación de la muestra.DISCUSIÓN: Nuestros resultados ofrecen unos buenos resultados en la validación de las muestras. La antigenicidad del tejido está conservada. Las muestras seleccionadas en el array representan alrededor del 97%, similar a todo el conjunto de las secciones convencionales de la muestra problema.PALABRAS CLAVE: Microarray. Cáncer vesical. Cáncer ureteral. FISH. Inmunohistoquímica. ABSTRACT HISTOPATOLOGICAL VALIDATION OF TISSUE-MICROARRAY TECHNOLOGY IN UROTELIAL CANCER. OUR EXPERIENCE INTRODUCTION:The array technology offers: a big advance to clinic and basic investigator, it provides a variety of technics (immunohystochemistry, FISH, proteomics) to undestand the molecular mechanisms of cancer. It offers scale economy in reagents versus the conventional methods. Array most be ratified because the sample is so reduced.MATERIAL AND METHODS: 52 consecutive cases have been cloosen from paraffin blocks of bladder and ureteral cancer which are 5-7 years old, a tissue array has been made; disks have been arranged in lines and columns, in an aleatory way, in order to guide it's reading. It has been evaluated by a pathologist with any relation to specimen selection.RESULTS: 87 sheets ha been obtained. Number 1 has been dyed with HE. Has been discrepancy in 27% of sample's stage. Has not been a discrepancy in hystopathologic diagnostic. There is no sample's representation in 11 points (17%).DISCUSSION: Our results offer good results in sample's validation. The sample's antigenicity of tissue is conserved. Array sample's represent a 97%, similary to all unit of conventional sections of the specimen.KEY WORDS: Microarray. Bladder cancer. Renal pelvis cancer. FISH. Immunohistochemistry.
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