Magnetic nanoparticles Fe3O4 were synthesized by the co-precipitation method. Magnetic nanoparticles were activated and modified with (3-aminopropyl) triethoxysilane (APTES) and glutaraldehyde (GA), respectively. Then, these nanoparticles were characterized by transmission electron microscope and Fourier transform infrared. Candida rugosa lipase (CRL) was successfully immobilized on GAMNPs by the chemical method via forming a covalent bond between a functional group on an enzyme protein molecule and a reactive group on the surface of solid support by chemical interaction. CRL-Fe3O4 displayed an elevate recovery rate of upward to 1348%, which was a 13-fold improvement in its free one. Compared to a free enzyme, the pH and thermal properties of the immobilized lipase were also increased. This study clearly indicates that GAMNPs could be deemed good support for the immobilization of enzymes.
Puree of potato was prepared with the addition of natural antioxidants such as carrots, chicken liver and underwent bacteriological and chemicals testing for the detection of anti-oxidative stress by assessing the fatty acid oleic and peroxide value and total nitrogen, the results showed that all of the carrot and liver individually contributed to the effectiveness of a good anti-oxidants liver carrot showed more effective in a good test for the peroxide oxidation and carrot showed more effectiveness in a good test in oleic as anti-oxidants and showed more effectiveness as good anti-oxidants in the test the total nitrogen.
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