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The present study aimed to explore changes in physiological and reproductive performances upon supplementation of +(-) cactechin (200 vs. 2000 mg/kg BW) in mice. In addition, body temperature, heart rate, blood oxygen and glucose were explored after general anesthesia of +(-)cactechin animals. Sixty albino female mice were classified into three groups; control group (G1) versus two +(-) cactechin groups [G2 (200 mg/kg BW) and G3 (2000 mg/kg BW)] for 15 days in aqueous solution. Changes of body temperature, heart rate, blood parameters, oocyte quality and reproductive performances were determined and recorded. In addition, changes of body temperature, heart rate, blood oxygen and glucose were measured after anesthesia. The results indicated that +(-)catechin supplementation resulted in significant increase in body temperature, blood oxygen and WBCs in addition to significant hypoglycemia. The oocytes quality and reproductive performances were improved upon +(-)catechin supplementation. +(-)catechin supplementation did not improve the negative side effects of anesthesia. In conclusion, supplementation of +(-)catechin could benefit physiological and reproductive performances in mice.

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Laying hens were employed to study the effects of carrier on the true metabolizable energy (TME) of tallow using corn, milo, soybean meal, wheat bran, and dehydrated alfalfa meal with tallow at 0, 2, 4, or 6%. In addition to bomb calorimetry measurements on feed and excreta samples, tallow TME values were calculated from absorbability data. Estimates of TME for tallow varied with carrier feedstuff from 7.19 kcal/g in soybean meal to 13.62 kcal/g when wheat bran was fed. For the corn, milo, and soybean meal series only fat retentions were significantly affected by tallow supplementation. Calculated tallow absorbabilities were 101.8% with corn, 95.8% with milo, and 107.3% with soybean meal. Highest tallow TME values were obtained with wheat bran and dehydrated alfalfa meal, apparently due to increase protein retention from wheat bran and to both improved protein retention and starch absorption with dehydrated alfalfa meal.

The present study aimed to explore the changes of body temperature, heart rate, blood partial pressure of oxygen (PO2) and glucose levels upon diazepam and/or xylazine administration in mice. Sixty male albino mice (33.89 ± 0.41 g body weight; BW) were distributed randomly into five groups given intraperitoneal (IP) injection: control group given physiological saline; diazepam group (D) given13.3 mg/kg BW; xylazine group (X) given26.6 mg/kg BW); DX group given both13.3 mg/kg BW diazepam and 26.6 mg/kg BW xylazine; DXVas group given DX dose for vasectomy surgical operation. The values of body temperature, PO2, heart rate and blood glucose were recorded of groups at 0, 20 min, 40 min, 1h, 2h, 3h, 4h, 8h of diazepam and/or xylazine injection. The results indicated a significant (P < 0.05) transient negative side effect of diazepam and/or xylazine dosages on body temperature, PO2, heart rate and blood glucose, which were the highest at 1-2 h post-drug administration. The transient negative side effects decreased gradually thereafter at 2h, 3h, 4h and 8hof drugs' injection. The transient negative side effects were more significantly pronounced in xylazine, DX and DXvas groups compared to diazepam and control groups and extended to 8h of injection. In conclusion, the given dosage of diazepam and/or xylazine resulted in transient negative side effects in body temperature, PO2, heart rate and blood glucose, which returned approximately close to normal levels at 8h of injection. The DX anesthesia dose is sufficient and safe for performing minor and major surgeries in mice.