The living tree sloths Choloepus and Bradypus are the only remaining members of Folivora, a major xenarthran radiation that occupied a wide range of habitats in many parts of the western hemisphere during the Cenozoic, including both continents and the West Indies. Ancient DNA evidence has played only a minor role in folivoran systematics, as most sloths lived in places not conducive to genomic preservation. Here we utilise collagen sequence information, both separately and in combination with published mtDNA evidence, to assess the relationships of tree sloths and their extinct relatives. Results from phylogenetic analysis of these datasets differ substantially from morphology-based concepts: Choloepus groups with Mylodontidae, not 64 Megalonychidae; Bradypus and Megalonyx pair together as megatherioids, while monophyletic Antillean sloths may be sister to all other folivorans. Divergence estimates are consistent with fossil evidence for mid-Cenozoic presence of sloths in the West Indies and an early Miocene radiation in South America. The sloths (Xenarthra, Folivora), nowadays a taxonomically narrow (6 species in 2 genera) component of the fauna of South and Central America 1,2 were once a highly successful clade of placental mammals as measured by higher-level diversity (Fig. 1). Diverging sometime in the Palaeogene from their closest relatives, the anteaters (Vermilingua), folivorans greatly expanded their diversity and range, eventually reaching North America as well as the West Indies 3-8. During the late Cenozoic sloth lineage diversity may have expanded and contracted several times 9. Final collapse occurred in the late Quaternary (end-Pleistocene on the continents, mid

The goal of this paper is to assess the variation in the proportional contribution of diverse resources to the diet of human populations from northwest Patagonia (Argentina) throughout the Middle-Late Holocene. Particularly, we assessed the variation among three geographic areas and two periods. We first estimated the expected proportions of terrestrial animals and plants and aquatic resources for each area according to the Binford's frames of references approach. A Bayesian mixing method was then applied to calculate the proportion of plants and animals in the diets from stable isotopes (δ 13 C and δ 15 N) of human bone collagen. The isotope values suggest that the composition of diets differed spatially and temporally. Diets of South Mendoza were mainly composed of terrestrial animals (Rhea-Lama and rodents) with a greater incorporation of C 3 plants towards the later Late Holocene; in North Neuquén, Rhea and Lama represent a proportion of 0.84 of the diet consumed; and finally, the sample of Center Neuquén is the only one with high values of Araucaria in the diet. The isotopic values obtained for the three studied areas did not fit to the expectations of Binford's model, North Neuquén being the area that departs most from the predicted proportions of terrestrial animals and plants and aquatic organisms in the diet. These findings open up new questions about the local conditions that influenced regional variation in the diet of prehistoric hunter-gatherers.

The study evaluates the psychometric properties of a Polish translation of the Brief Illness Perception Questionnaire. A total of 276 patients with chronic conditions (58.7% women) completed the Brief Illness Perception Questionnaire and the Hospital Anxiety and Depression Scale. The internal consistency of the Polish Brief Illness Perception Questionnaire measured with Cronbach's alpha was satisfactory (α = 0.74). Structural validity was demonstrated by significant inter-correlations between the Brief Illness Perception Questionnaire components. Discriminant validity was supported by the fact that the Brief Illness Perception Questionnaire enables patients with various conditions to be differentiated. Significant correlations were found between Brief Illness Perception Questionnaire and depression and anxiety levels. The Polish Brief Illness Perception Questionnaire thus evaluated is a reliable and valid tool.

An extensive in situ Raman spectroscopic campaign was performed on archaeological sites in three different provinces in Patagonia, Argentina (Neuquén, Río Negro and Chubut). 16 open air shelters located in different environments (forests, ecotones, steppes) were investigated and interpreted in terms of pigments used and the identification of substrata. Special attention was given to the alteration products and accretions that were found on the rock art paintings of the shelters and on the surface of the rock walls, as they can affect and damage this magnificent works of art. Haematite (α-Fe 2 O 3) was the main chromophore that was found on the red paintings of the most of the shelters studied. The green earth glauconite, was identified only in one case, by using a red (785 nm) and a green laser (532 nm). Other minerals and silicates were found on the couloured areas but also on the rock support. Calcite (CaCO 3) and gypsum (CaSO 4 •2H 2 O) crystallization was identified on the paintings, crusts and rock surfaces, in combination or alone, and are associated with weathering. In some cases the shelters were so severely degraded that no Raman signal of pigments and/or other components could be retrieved. Calcium oxalates were also detected in several figures and motifs in different shelters.

Objectives Illness perception is a cognitive representation influencing physical and psychological functioning and adherence in patients with rheumatic disease. Studies exploring illness perception in systemic lupus erythematosus (SLE) are still scarce and none of them have investigated factors determining illness perception. We aimed to assess illness perception and to identify psychological, clinical and sociodemographic factors that might influence illness perception in SLE. Methods The study involved 80 patients with SLE (87.5% women, mean age 41.56 years). The Brief Illness Perception Questionnaire, State Trait Anxiety Inventory, Beck Depression Inventory, Pittsburgh Sleep Quality Index, Visual Analogue Scale-Pain and Fatigue Severity Scale were used. Clinical and sociodemographic data were collected via structured interview and medical files review. Results Illness perception was significantly positively correlated with anxiety, depression, sleep quality, fatigue and pain while it was not related to age, education, steroid treatment, disease duration and activity (SLEDAI) or organ damage (SLICC/ACR). Regression analysis revealed that state anxiety and depression explained 43% of illness perception variance. Cluster analysis identified three patient groups among which the middle-aged group had the most negative illness perception, the highest levels of anxiety, depression, pain and fatigue, and the poorest sleep quality. Conclusions The study has proved a significant relationship between negative illness perception and anxiety and depression. Patients reporting fatigue, poor sleep and pain might have special needs in terms of psychological intervention focused on negative illness perception and distress symptoms. Multidisciplinary care in managing SLE seems to be of great importance.