Acidification (OA), a global threat to the world’s oceans, is projected to significantly grow if CO2 continues to be emitted into the atmosphere at high levels. This will result in a slight decrease in pH. Since the latter is a logarithmic scale of acidity, the higher acidic seawater is expected to have a tremendous impact on marine living resources in the long-term. An 8-week laboratory experiment was designed to assess the impact of the projected pH in 2100 and beyond on fish survival, health, growth, and fish meat quality. Two projected scenarios were simulated with the control treatment, in triplicates. The control treatment had a pH of 8.10, corresponding to a pCO2 of 321.37 ± 11.48 µatm. The two projected scenarios, named Predict_A and Predict_B, had pH values of 7.80-pCO2 = 749.12 ± 27.03 and 7.40-pCO2 = 321.37 ± 11.48 µatm, respectively. The experiment was preceded by 2 weeks of acclimation. After the acclimation, 20 juvenile black sea breams (Acanthopagrus schlegelii) of 2.72 ± 0.01 g were used per tank. This species has been selected mainly due to its very high resistance to diseases and environmental changes, assuming that a weaker fish resistance will also be susceptibly affected. In all tanks, the fish were fed with the same commercial diet. The seawater’s physicochemical parameters were measured daily. Fish samples were subjected to physiological, histological, and biochemical analyses. Fish growth, feeding efficiency, protein efficiency ratio, and crude protein content were significantly decreased with a lower pH. Scanning electron microscopy revealed multiple atrophies of microvilli throughout the small intestine’s brush border in samples from Predict_A and Predict_B. This significantly reduced nutrient absorption, resulting in significantly lower feed efficiency, lower fish growth, and lower meat quality. As a result of an elevated pCO2 in seawater, the fish eat more than normal but grow less than normal. Liver observation showed blood congestion, hemorrhage, necrosis, vacuolation of hepatocytes, and an increased number of Kupffer cells, which characterize liver damage. Transmission electron microscopy revealed an elongated and angular shape of the mitochondrion in the liver cell, with an abundance of peroxisomes, symptomatic of metabolic acidosis.
This work investigates the effect of low frequency vibratory processing for cleaning and washing various machine components parts from rusts and old paints deposits. The experimental investigation was carried out with special prepared samples that were weighted and exposed to paints and rust contaminants. These samples were treated in universal horizontal vibration machine UVHM 4 × 10 with different combination of instrumental processing medium, process fluid, machine amplitude and frequency of oscillations. They were periodically reweighted after processing and compared to etalon with control of quantity of dust that have been removed, sample cleanliness and also other functional parameters. Statistical analysis has been used to characterize ongoing process and full factorial analysis to establish experimental parameters dependency. The result is showing the complex dependence of samples cleanliness to each processing parameters like processing time, amplitude of oscillations, frequency of oscillations, process fluid parameters, instrumental medium, etc. Between this parameters although the most important successively the amplitude of oscillations, the frequency of oscillations the processing medium and the processing fluid depending to his considered composition, the optimal processing time can be reach only by complex combination of all this parameters every of them carry an amplify coefficient. Low frequency oscillations can be used to monitor and optimize washing and cleaning operations of paints and rusts contaminations. That guarantees process automation, its effectiveness for a large industrial application.
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