Background Different types of dietary lipids have been shown to affect lipid metabolism and lipid profile differently. Objective This study aims to assess the effect of local niger seed oil on serum lipid profile compared to palm oil and sunflower oil in rats. Methods The effect of the 15% plant oils on serum lipid profile, body weight gain percentage, and feed efficiency ratio was assessed after 8 weeks of experimental period. Results and Conclusion The 15% niger seed oil showed decrease and increase in the level of lipid profile as compared to rats fed with 15% palm oil and sunflower oil (except Triacylglycerol), respectively. The 15% niger seed oil showed significant decrease and increase in body weight gain percentage as compared to the 15% palm oil and 15% sunflower oil, respectively. The feed efficiency ratio was significantly higher and lower in the 15% niger seed oil compared to rats fed with 15% sunflower oil and control group and the palm oil fed rats, respectively. The current study concluded that consumption of locally manufactured niger seed oil decreased the blood lipid profiles, body weight gain percentage, and feed efficiency ratio as compared to palm oil. Utilization of oils containing more unsaturated fatty acids like niger seed oil is recommended to reduce the risk of developing cardiovascular disease.