MATERIAL AND METHODSDuring necropsy, 53 fragments of muscles were excised from 41 corpses, less than 24 hours after death. Os these samples, 29 were of anterior tibial muscle (26 males and 3 females: 19 white, 9 mulattoes and 1 negro, ages varying from 4 to 63 years); the remaining 12 samples comprised deltoid and pectoral major muscles (10 males and 2 females: 7 white and 5 mulattoes, ages ranging from 9 to 60 years).The causes of death included: craniocerebral trauma of several types (21 cases), polytrauma (9), drowning (4), post-traumatic internal hemorrhage (4), heart attack (1), asphyxia (1) and lesions in the neck produced by shot-gun (1).The muscle samples were dried in an oven at 105°C until constant weight. These dry tissues were incinerated in a furnace regulated potentiometrically at 550 D C during approximately 12 hours (overnight). Ash solutions were prepared using 2N hydrochloric acid.
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