Efficient and reproducible construction of signaling and sorting complexes, both on the surface and within the living cell, is contingent on local regulation of biochemical reactions by the cellular milieu. We propose that in many cases this spatiotemporal regulation can be mediated by interaction with components of the dynamic cytoskeleton. We show how the interplay between active contractility and remodeling of the cytoskeleton can result in transient focusing of passive molecules to form clusters, leading to a dramatic increase in the reaction efficiency and output levels. The dynamic cytoskeletal elements that drive focusing behave as quasienzymes catalyzing the chemical reaction. These ideas are directly applicable to the cortical actin-dependent clustering of cell surface proteins such as lipid-tethered GPI-anchored proteins, Ras proteins, as well as many proteins that have domains that confer the ability to interact with the actin cytoskeleton. In general such cytoskeletal driven clustering of proteins could be a cellular mechanism to spatiotemporally regulate and amplify local chemical reaction rates in a variety of contexts such as signaling, transcription, sorting, and endocytosis.active hydrodynamics | signal transduction

We investigate the steady state heat current in two and three dimensional disordered harmonic crystals in a slab geometry, connected at the boundaries to stochastic white noise heat baths at different temperatures. The disorder causes short wavelength phonon modes to be localized so the heat current in this system is carried by the extended phonon modes which can be either diffusive or ballistic. Using ideas both from localization theory and from kinetic theory we estimate the contribution of various modes to the heat current and from this we obtain the asymptotic system size dependence of the current. These estimates are compared with results obtained from a numerical evaluation of an exact formula for the current, given in terms of a frequency transmission function, as well as from direct nonequilibrium simulations. These yield a strong dependence of the heat flux on boundary conditions. Our analytical arguments show that for realistic boundary conditions the conductivity is finite in three dimensions but we are not able to verify this numerically, except in the case where the system is subjected to an external pinning potential. This case is closely related to the problem of localization of electrons in a random potential and here we numerically verify that the pinned three dimensional system satisfies Fourier's law while the two dimensional system is a heat insulator. We also investigate the inverse participation ratio of different normal modes.

This paper investigates the uncertainties related to atmospheric fields from reanalysis products used in forcing ocean models. Four reanalysis products, namely from 1) the interim ECMWF Re-Analysis (ERA-Interim), 2) version 2 of the Common Reference Ocean–Ice Experiments (CORE2), 3) the 25-Year Japanese Reanalysis Project (JRA-25), and 4) NCEP–NCAR, are evaluated against satellite-derived observations for eight different fields (zonal and meridional winds, precipitation, specific humidity, continental discharge, surface air temperature, and downwelling longwave and shortwave radiation fluxes). No single product is found to agree better in all fields with satellite-derived observations. Reanalysis products are mostly comparable to each other because of their similar physical assumptions and assimilation of common observations. Adjusted atmospheric fields from the Estimating the Circulation and Climate of the Ocean (ECCO) optimizations are also in agreement with other reanalysis products. Time-mean and time-variable errors are estimated separately and mapped globally in space, based on 14-day average fields to focus on monthly to interannual periods. Time-variable errors are larger in comparison to the signal than time-mean errors for most fields, thus justifying the need to separate them for studying uncertainties as well as formulating optimization procedures. Precipitation and wind stress fields show significant time-mean and time-variable errors whereas downwelling radiation, air temperature, and humidity fields show small time-mean errors but large time-variable errors, particularly in the tropics. Uncertainties based on evaluating multiple products presented here are considerably larger than uncertainties based on single product pairs.