: Urinary tract infection is one of the most common infectious disease found in all population and has become a major health problem. The urinary tract infection also affects pediatric population especially in the age group of 0-5 years. The aim of this study to find out prevalence of UTI in children, its occurrence in different gender and seasons along with identification of causative agent of UTI in children in Ujjain. The study was carried out in SRL laboratory Ujjain center and total 881 suspected patients urine sample were collected and tested by conventional methods for prevalence of significant bacteriuria. It was seen that 550 samples were positive and out of these, positive case 111 belonged to children of 0-5 years of age. Thus, 20.18% positive cases were of children. The percentage of UTI in female children was high (54.0%) while in male children it was low (45.9%) and high number of cases were recorded in rainy season (39.6%), followed by summer and winter seasons which were (34.2%) and (26.1%) respectively. The six major uropathogens present in urine sample were isolated and identified and among them four were gram negative in which . , . , . , . were gram negative while and . and . were gram positive UTI causing bacteria in children. In our study frequency of . was highest (60.3%) while . was present in very low frequency (3.6%) so the prevention of UTI in children should be done by proper care of children by mothers.
Background: Nonfermentative gram negative bacilli (NFGNB) frequently considered as commensals or contaminants but the pathogenic potential of nonfermenters has been proved beyond doubt. They are resistant to commonly used antimicrobials. Aim: This study was undertaken to identify the nonfermenters isolated from various clinical samples and to know their Antibiotic sensitivity pattern. Materials and Methods: The present study was carried out on 150 strains of Nonfermenters isolated from 1200 various non repetitive clinical samples received in Department of Microbiology, NIMS Jaipur. Nonfermenters were identified using a standard protocol and their antibiotic susceptibility testing was performed with the help of the modified Bauer disc diffusion method. Results: Out of 150 nonfermenters isolated, Pseudomonas aeruginosa was the most common isolate 134 (89.33%) followed by Acinetobacter baumannii 16 (10.67%). Among all clinical samples Pus and Wound Discharge yield maximum isolates of NFGNB i.e. 54 (36%) % followed by sputum (39.0%). Most sensitive drug against NFGNB was Polymyxin-B (100%) followed by Imipenem (86 %) and Amikacin (71.33 %). Conclusion: Nonfermenters have a great potential to survive in a hospital environment so implementation of antibiotic stewardship programs and strict infection control practices will be required to prevent or slow down their emergence and spread. Keywords: Nonfermenters, Polymyxin-B, Pseudomonas, Acinetobacter.
Background: Non fermentative gram negative bacilli that were considered to be contaminants in the past have now emerged as important health care associated pathogens. Aim: This study was undertaken to identify and characterize Nonfermenters isolated from various clinical samples. Material and Methods: Present study was conducted in the department of Microbiology, National Institute of Medical Sciences (NIMS) Medical College and hospital, Jaipur from July 2013 to July 2014. A total 150 strains of Nonfermenters were isolated from 1200 various non repetitive clinical samples. The samples were processed and Non fermentative gram negative bacilli (NFGNB) identified as far as possible up to species level as per standard protocols. Results: In the present study, 12.50% of incidence / isolation rate of Nonfermenters in various clinical samples was found. Out of 150 strains of Nonfermenter, Pseudomonas aeruginosa was the most common isolate 134 (89.33%) followed by Acinetobacter baumannii 16 (10.67%). 89 (59.33%) NFGNB were isolated from males and 61 (40.67%) were from females. They were mainly isolated from age group 21-30 Years. Higher rate of isolation of NFGNB were 85 (56.67%) from IPD and 38 (25.33%) from ICU patients. Among all clinical samples Pus and Wound Discharge yield maximum isolates of NFGNB i.e. 54 (36%) followed by Sputum 39 (26.0%). Conclusion: NFGNB should not be ignored as mere contaminant but correlate clinically for its pathogenic potential and identified using standard protocol so as to institute appropriate and timely antibiotic coverage Keywords: Identification, Nonfermenter, Pseudomonas, Acinetobacter
Urinarytract infection is one of the most prevalent frequently found disorders in pregnant women caused by bacteria and if it is not diagnosed then untreated UTI can causes serious maternal and fetal complications. The purpose of this work was to study the prevalence of urinary tract infection among pregnant women of Ujjain and to identify uropatogens and their antibiotic susceptibility pattern in pregnant women of Ujjain. A total of 120 midstream urine samples of pregnant women were collected aseptically and cultured on different growth media. The isolated bacteria were identified by standard microbiological procedure and the antibiotic susceptibility pattern was recorded using Kirby-Bauer disc diffusion method. Out of 120 urine samples, 78 samples tested positive for UTI suggesting that (65%) of pregnant women suffered from UTI and most prevalent bacterial pathogen was (41.0%) followed by (23.0%), (11.5%), (14.1%) and (10.2%). Majority of the isolated uropathogens were showing high sensitivity towards Doripenem, Meropenem and Imipenem while very less sensitivity towards Ampicillin and Penicillin. The study shows high prevalence of UTI among pregnant women and occurrence of high resistance towards antibiotics. So routine screening of the pregnant women having UTI and proper selection of antibiotic for treatment is needed for reducing the risk of serious complications during pregnancy.
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