Aim:The present study was carried out to compare the associated role of micro minerals and hormones in repeat breeding animals with the normal crossbred cows.Materials and Methods:Blood samples were collected from 10 normal cycling and 10 repeat breeding crossbred cows of Ramakrishna Mission Ashram, Narendrapur to study the plasma mineral profile and hormonal activities.Results:Zn was found to be highly significant (p<0.01) between the two groups. Follicle stimulating hormone (FSH) and progesterone showed significant (p<0.05) difference in repeat breeding animal from the normal cyclic animal, whereas no significant differences were observed in Ca, P, Cu, Se, Co, luteinizing hormone and estradiol level.Conclusion:It may conclude that repeat breeding condition of crossbred cows in farm condition is mainly due to the low level of progesterone, FSH and zinc.
Jamunapari is one of the largest and most popular goat breeds in India. The present study was carried out to purify and characterize the IgG of Jamunapari goat since IgG plays central role in the fight against infectious diseases. The IgG of Jamunapari goat could be isolated by salting out (50% saturated ammonium sulfate solution) and purified by the gel filtration chromatography on Sephacryl S-200. The Jamunapari goat immunoglobulin G (IgG) was obtained in relatively purified form. The purified Jamunapari goat IgG revealed major bands of 61 kDa, 55 kDa, suggestive of heavy chains and 28kDa, 23 kDa, suggestive of light chains. The purified immunoglobulin has been found to be immunoreactive by DID and western blot analysis in the present study.
The caseins (Alpha s1, Alpha s2, beta and kappa) comprise the major protein component of ruminant milk and are secreted in the form of stable calcium phosphate micelles. The present study was proposed to characterize αs1-casein gene in buffalo at the molecular level to determine complete αs1-casein cDNA sequence. Sequencing results were analyzed and aligned with available mRNA GenBank sequences of other domestic animals (Accession AAB34797, NM_001009795, X72221, AY344966, NM_181029, AY948385 and BC109618). The complete amino acid coding region of the buffalo αs1- casein was deduced from cloned cDNA generated from mammary tissue mRNA. Amino acid sequence comprises of 199 amino acid residues. When aligned with previously reported buffalo sequence (Accession-AY948385) it showed one amino acid (Glutamine) deletion at amino acid position 78.
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