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The detection of cortisol in saliva is an important screening tool for psychological stress and health monitoring, including the diagnosis of Cushing's syndrome, Addison's disease, and post-traumatic stress disorder (PTSD). In this work, a simple, low-cost, label-free, electrochemical immunosensing platform is explored for highly sensitive and selective detection of cortisol in saliva. Anti-Cortisol antibodies (Anti-C ab ) covalently immobilized on self-assembled monolayer (SAM) of dithiobis(succinimidylpropionte) (DTSP) modified microfabricated interdigitated microelectrodes (IDEs) were used for electrochemical detection of cortisol. The non-binding sites of immunosensor surface were blocked using ethyleneamine (EA). Electrochemical response studies as a function of cortisol concentrations were conducted using cyclic voltammetry (CV). The sensor exhibited a detection range from 10 pg/mL to 100 ng/mL, a detection limit of 10 pg/mL, and a sensitivity of 6 μA/(pg/mL) with the regression coefficient of 0.99. The obtained sensing parameters were in physiological range. The sensor was successfully tested on multiple specimen samples of saliva collected at different time intervals from two participants. The obtained cortisol concentrations from the developed electrochemical system correlate well with those were obtained using ELISA.) unless CC License in place (see abstract). ecsdl.org/site/terms_use address. Redistribution subject to ECS terms of use (see 199.212.65.7 Downloaded on 2014-11-04 to IP B3078Journal of The Electrochemical Society, 161 (2) B3077-B3082 (2014) ) unless CC License in place (see abstract). ecsdl.org/site/terms_use address. Redistribution subject to ECS terms of use (see 199.212.65.7 Downloaded on 2014-11-04 to IP ) unless CC License in place (see abstract). ecsdl.org/site/terms_use address. Redistribution subject to ECS terms of use (see 199.212.65.7 Downloaded on 2014-11-04 to IP ) unless CC License in place (see abstract). ecsdl.org/site/terms_use address. Redistribution subject to ECS terms of use (see 199.212.65.7 Downloaded on 2014-11-04 to IP

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Articles you may be interested inA novel flexure-based microgripper with double amplification mechanisms for micro/nano manipulation Rev. Sci. Instrum. 84, 085002 (2013); 10.1063/1.4817695 Electrowetting on dielectric-based microfluidics for integrated lipid bilayer formation and measurement Appl. Phys. Lett. 95, 013706 (2009); 10.1063/1.3167283 Development of novel hybrid flexure-based microgrippers for precision micro-object manipulation Rev. Sci. Instrum. 80, 065106 (2009); 10.1063/1.3147062Electrowetting-based valve for the control of the capillary flow

We present a low-voltage microgripper utilizing a liquid droplet to pick up and release micro-objects. Lifting force is generated by a liquid bridge formed between the gripper surface and an object. Electrowetting was used to dynamically change the capillary lifting forces and enable easy object release. The driving voltage was applied to a pair of coplanar interdigitated electrodes, eliminating the need for an electrode on top of the droplet and thus significantly facilitating object manipulation. A barium strontium titanate insulation layer was used to lower the driving voltage. Experiments indicated that the lifting forces can be as high as 213 μN at a driving voltage of 28 V. Experiments also demonstrated a low-voltage, low power consumption soft microgripper by picking up and releasing micro glass beads.