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Camelina sativa is an oilseed crop of the Brassica (Cruciferae) family that has gained increased popularity as a biofuel source. The present study was conducted to investigate the effects of feeding C. sativa meal to broiler birds on phenolic compounds, tocopherols, flavonoids, antioxidant capacity, and lipid peroxidation in chicken thigh meat during short (4°C for 2 or 7 d) or long-term (-20°C for 90 d) storage and cooking. One hundred sixty 1-d-old Cobb chicks were fed a corn-soybean meal-based diet with added Camelina meal at 0% (control), 2.5% (CAM2.5), 5% (CAM5), and 10% (CAM10). The experimental diets were fed for a period of 42 d. Feeding Camelina meal at 5 or 10% led to a 1.6-fold increase in γ-tocopherols in the thigh meat when compared with control birds (P < 0.05). No effect of diet on γ-tocopherols in the breast meat and α-tocopherols in the thigh and breast meat was observed (P > 0.05). Antioxidant activity measured as 2,2-azino-bis [3-ethylbenzo-thiazoline-6-sulfonic acid] radical scavenging capacity in the thigh meat from CAM2.5, CAM5, and CAM10 was higher than control birds (P < 0.05). In the breast meat, 2,2'-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt radical scavenging capacity was highest in CAM10 (P < 0.05). Feeding Camelina meal at 5 or 10% led to reductions in thigh TBA reactive substances (TBARS) during short-term (2 d) and long-term storage (P < 0.05). The TBARS of thigh meat from CAM5 and CAM10 were reduced up to 49 or 36% during 2- or 90-d storage, respectively, when compared with control (P < 0.05). However, no effect of diet on thigh meat TBARS at 7 d of storage was observed among treatment groups. Overall, TBARS were highest in the thigh meat from control and CAM2.5 birds (P < 0.05). Upon cooking, TBARS were lowest in thigh meat from CAM10 birds, which was over 48% lower than in meat from birds fed the control diet (P < 0.05). The current study showed that Camelina meal could be effective in inhibiting lipid oxidation and enhancing antioxidant capacity. However, the effect was more prominent in the thigh than breast meat.

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An experiment was conducted to determine the impact of using garlic and ginger powder on growth performance, body composition, lipid peroxidation and antioxidant enzymes activities in muscle tissues of Nile tilapia fingerlings. Three isonitrogenous (32%) and isocaloric (3000 kcal DE) diets were formulated , control basal diet, diet supplemented with 1.5% ginger powder, other diet supplemented with 1.5% garlic powder and fed to the fish for sixty days at 3% body weight. No significant effects were found in final body weight (FBW) between experimental groups of fish. Body weight gain (BWG) and specific growth rate (SGR) were significantly (p≤ 0.05) decreased in Nile tilapia fish fed diets supplemented with garlic and ginger powder compared to the control group. Also, there was improvement of feed conversion ratio (FCR) of Nile tilapia fish fed control basal diet compared with other experimental groups. No significant differences in proximate chemical composition of whole body of fish between experimental groups. Lipid peroxidation (malondialdehyde, MDA) in muscle tissues of fish groups fed diets supplemented with ginger and garlic (1.5%), respectively, showed a significant (p≤ 0.05) decrease in MDA levels. Also, superoxide dismutase (SOD) was significantly (p≤ 0.05) increase in fish group fed diet supplemented with garlic compared with other experimental groups. No significant differences of Catalase (CAT) and reduced glutathione (GSH) of fish muscle of experimental groups. To sum up, adding garlic and ginger at 1.5% had no significant effect on Nile tilapia growth performance, body composition, while using of garlic as a feed additive significantly reduce lipid peroxidation and had antioxidant effect.

Objective: To evaluate the effect of dietary supplementation of essential oils (EOs) of thyme, clove, and cinnamon to broiler chickens on growth performance, serum metabolites, and tissue lipid peroxidation and antioxidants enzyme activities. Design: Randomized controlled study Animals: One hundred-eighty, one-day-old Cobb broiler chicks were randomly allocated into 4 treatment groups (3 replicates, 15 chicks each). Broilers were reared in deep litter system - pens for 6 weeks of age. Procedures: The control group was fed on corn-soybean basal control diets (starter, grower and finisher) without EOs supplementation. In addition to the basal- control diets, the 3 experimental broiler groups were supplemented with thyme oil (2nd group), clove oil (3rd group) or cinnamon oil (4th group) at 100 mg/kg. Blood, liver and muscle (breast) samples were collected from 3 broilers of each replicate at 40 days of age for measurement of serum metabolites, malondialdehyde (MDA), and antioxidant enzyme activities (liver catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Results: The broiler chickens fed on the diets supplemented with 100 mg/kg cinnamon oil had significantly higher body weight, body weight gain and feed intake, followed by broiler chickens fed on the diets supplemented with 100 mg/kg thyme and 100 mg/kg clove oils in comparison with control group, which was showed the lowest growth performance. Supplementation with EOs significantly improved feed conversion ratio (FCR), with increased liver CAT, SOD, GSH-Px activities, as well as serum level of high density lipoprotein (HDL) in comparison with control group, also, it induced a significant decrease in serum levels of cholesterol, triglycerides, low density lipoprotein (LDL), as well as both liver and muscle tissue lipid peroxidation (MDA). Conclusion and clinical relevance: The present results indicate that dietary supplementation of EOs (thyme, clove, and cinnamon) at 100 mg/kg resulted in significantly higher body weight, body weight gain, improved FCR, reduced serum cholesterol, triglycerides and LDL. Also, reduced lipid peroxidation in liver and muscle, and improved antioxidants status of broiler chickens.

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Choline is an essential nutrient in laying hen diets and is needed for the formation of phosphatidylcholine ( PC ), that serves as a rich source of long chain (≥20 C) n-3 fatty acids ( FA ) in eggs. Methionine ( Met ) is the first limiting amino acid in layer hen diets and serves as a lipotropic agent with antioxidant properties. The objectives of the current study is based on the hypothesis that choline and Met supplementation will enhance egg PC and n-3 FA status, lipid stability, and production indices in layer hens fed flaxseed. Ninety-six, 40-wk-old laying hens (W-36 White Leghorns) were randomly allocated to 4 treatment groups, with 6 replicates containing four hens per cage. Hens were fed corn-soybean meal-based diet containing 0% flaxseed ( Control ), 15/100 g flaxseed ( Flax ), Flax+50% more methionine requirement for W-36 White Leghorns ( Flax+Met ), or Flax+0.15g/100g choline chloride ( Cho ) ( Flax+Cho ). All experimental diets were isocaloric and isonitrogenous and fed for a period of 120 d. Egg production and egg mass (g/hen/d) was higher for Flax+Met and Flax+Cho when compared to Flax and Control ( P < 0.05). Egg weight was greater ( P < 0.05) among hens fed the Control and Flax+Cho diets compared to Flax diet. Feeding flaxseed to hens led to over 6-fold increase in total n-3 FA. Choline supplementation increased egg α-tocopherol content ( P < 0.05) while reducing lipid oxidation products measured as thiobarituric acid reactive substances in egg yolk ( P < 0.05). Neither Met nor Cho had any impact on docosahexaenoic (22:6 n-3) acid concentration in eggs from hens fed flaxseed. However, addition of Met and Cho to layer diets increased docosapentaenoic acid (22:5 n-3) levels in eggs from hens fed flaxseed ( P < 0.05). The PC content was lower in Control and Flax+Met ( P < 0.05) when compared to Flax+Cho group. No difference was found in total lipid or phosphatidylethanolamine content of eggs ( P > 0.05). The results from the current study suggest that n-3 FA content of egg yolk can be greatly increased by feeding flaxseed but reduced egg production. However, dietary Met and Cho can improve production performance in hens fed flaxseed-containing diets. Addition of Cho to flaxseed increased in egg weight, yolk α-tocopherol levels, PC content and oxidative stability of eggs when compared to hens fed flaxseed. Met and choline could be used in flaxseed (>15%) to increase egg production and egg mas.