The pollution of agricultural soils, water and plants by trace elements (TEs) in the Nile Delta Region, Egypt, is of great importance. This study aimed to investigate the spatial and seasonal variation of some TEs in the agricultural area adjacent to Kitchener Drain and to evaluate the ecological risk posed by these elements using six indices. Soil and plant samples were collected from seven sites close to the drain, while water samples were collected from the corresponding sites inside the drain during three seasons (winter, spring and fall). The results showed that all studied TEs in the soil varied seasonally and spatially among the locations around the drain. Most of the studied elements in the soil were higher in the southern and middle area around the drain. All studied elements in the soil were also higher in the winter than other seasons. Nickel and lead were almost non-detected during all seasons in plant tissues, while other elements were higher in the winter than other seasons. In contrast to the soil and plant tissues, water samples demonstrated lower or non-detected levels of TEs. The results also indicated that the values for the risk assessment indices differed among the studied TEs. Therefore, there is a risk of increasing the concentration of some metals in the study area due to anthropogenic pollution from the adjacent polluted drain through irrigation with contaminated water and spreading of contaminated dredged materials on agricultural fields.