Kontrol mutu hasil tangkapan nelayan di Pelabuhan Perikanan Branta, Pamekasan masih belum dilaksanakan dengan baik. Penelitian ini bertujuan untuk menentukan kualitas daging ikan kurisi berdasarkan nilai Total Plate Count (TPC), keanekaragaman jenis, total kelimpahan jenis bakteri heterotrof dan patogen pada media TSA, EMB, SSA, dan TCBS. Nilai TPC bakteri heterotrof (TSA) adalah 8,59 Log CFU/g dengan 7 keanekaragaman jenis dan total kelimpahan tertinggi antara 2-336 koloni (BH3). Nilai TPC bakteri patogen (EMB) adalah 3,72 Log CFU/g dengan 6 keanekaragaman jenis dan total kelimpahan tertinggi antara 784-1009 koloni (BPE4), serta isolat bakteri BPE1 yang berwarna hijau metalik diduga sebagai Escherichia coli. Nilai TPC bakteri patogen (SSA) adalah 4,12 Log CFU/g dengan 5 keanekaragaman jenis dan total kelimpahan tertinggi antara 35-450 koloni (BPS1), serta isolat bakteri BPS1 yang berwarna hitam diduga sebagai Salmonella sp. Nilai TPC bakteri patogen (TCBS) adalah 5,41 Log CFU/g dengan 2 keanekaragaman jenis dan total kelimpahan tertinggi antara 0-44 koloni (BPT1). Isolat bakteri BPT1 dan BPT2 yang berwarna hijau dan kuning diduga sebagai Vibrio parahaemolyticus dan Vibrio vulnificus.
Probiotik merupakan mikroba hidup dengan sifat menguntungkan. Jumlah air limbah cucian beras sangat melimpah dan memiliki kandungan nutrisi yang cukup banyak, terutama karbohidrat sebesar 85-90%. Air limbah kulit bawang putih berfungsi untuk menghambat pertumbuhan bakteri seperti Escherichia coli, Staphylococcus aureus, dan Salmonella enteritidis. Daun kelor (Moringa oleifera) merupakan tumbuhan yang mempunyai kandungan protein sebesar 27-29%. Tujuan penelitian ini adalah mengevaluasi kombinasi terbaik probiotik yang dapat digunakan dalam budidaya perikanan guna meningkatkan pertumbuhan dan kualitas ikan. Penelitian ini menggunakan rancangan acak faktorial (RAF) 4x4 dengan 2 faktor. Faktor pertama berupa kultur fermentasi ekstrak daun kelor yang terdiri atas 4 perlakuan, yaitu 10%; 20%; 30%; dan 40% dengan kombinasi antara air limbah cucian beras dan air limbah kulit bawang putih sebesar 1:1. Faktor kedua berupa lama waktu fermentasi probiotik yang terdiri atas 4 perlakuan, yaitu 1; 2; 3; dan 4 minggu. Kontrol negatif berupa air limbah cucian beras dan kulit bawang putih dengan perbandingan 1:1 dan kontrol positif dengan penambahan probiotik EM4 berkonsentrasi 10%. Setiap perlakuan diuji menggunakan 3 pengulangan. Metode penelitian ini menggunakan metode observatif. Hasil penelitian menunjukkan bahwa probiotik ikan yang diamati menghasilkan pelet pada permukaan dasar wadah fermentasi, gelembung gas, perubahan warna menjadi cokelat kuning, perubahan bentuk wadah fermentasi, bau khas seperti bau tape, dan tidak adanya kontaminasi jamur. Probiotics are a living microbe with a beneficial nature. Rice dishwater is plentiful and has a good amount of nutrition, especially a carbohydrate of 85-90%. Garlic skin wastewater helps prevent the growth of bacteria such as Escherichia coli, Staphylococcus aureus and Salmonella enteritidis. Kelor (Moringa oleifera) is a plant that has a protein content of 27-29%. The aim of this study is to evaluate the best possible combination of probiotics that can be used in fisheries cultivation to promote the growth and quality of fish. The study uses randomly vectorial design 4x4 with 2 factors. The first factor is the four treatments fermentation culture extract of kelor leaves, which is 10%; 20%; 30%; and 40% by the combination of rice dishwater and garlic wastewater by 1:1. The second factor is long-term probiotic fermentation, consisting of 4 treatments, 1; 2; 3; and 4 weeks. The negative control of rice and garlic skin dishwater by comparison 1:1 and positive control with an additional probiotic EM4 concentrates 10%. Each treatment is tested using three repetitions. This research method using observative. Research indicates that probiotic fish observed produce pellets on the ground surfaces of fermentation containers, gas bubbles, discoloration to yellow chocolate, fermentation containers change in shape, typically smells like tape, and an absence of fungal contamination.
Introduction/Main Objectives: This research integrates the self-categorization and contagion theories to analyze faultlines due to a decentralization system. Besides, this research investigates the inducement of tournament incentives as a control mechanism to mitigate the harmful effects of a faultline on group performance. Background Problems: Fiscal decentralization has a crucial role as it stimulates economic growth, enhances the quality of decision-making and escalates performance. However, decentralization by one local government, which consists of various local government departments, may trigger a faultline. This research argues that patterned diversity convenes faultlines that split up a group into antagonistic sub-groups following the attributes affecting the aggregate group performance. Novelty: This research provides a new insight, in that decentralization appears to be a double-edged sword. It can elevate the quality of local decision-making, and trigger faultlines between local government departments at other times, affecting the local government’s aggregate performance. Research Methods: This research uses a laboratory experimental method with a 2×3 between-subjects factorial design. The research design uses the dyad analysis level. Finding/Results: The results found that the induction of a tournament scheme with the use of a cumulative ordinal scale for determining group performance encourages the social cognitive activation of individuals, thus encouraging cognitive orientation to optimize compensation and minimize categorization and antagonism. Conclusion: A tournament incentive scheme can be induced as a management control mechanism and to encourage the sub-groups to be winners. This resolution is expected to mitigate antagonistic behavior due to faultlines and enhance the optimization of aggregate performance
Fish is a source of animal protein which is generally the choice of society because it is relatively cheap and easy to obtain. Fish that are sold in dead condition are prone to quality degradiation so that bacteria can easly breed. Data on the amount of bacteria in fish related to the type of gear to another which can greatly affect the quality of the catch. Therefore, this research was conducted using a Minitrawl. The research objective was to see the amount of bacteria in fish caught using a mini fishing boat before being given and after being notified by considering the amount of bacteria in the substrate in the waters, sea water and ice cubes used by fishermen. The TPC (Total Plate Count) research method using Tryptic soy Agar (TSA) as media and pathogenic bacteria using Thiosulfate Citrate Bile Salt Sucrose Agar (TCBS) as media on fish “kardinal pita lebar” (Apogon fasciatus), sea water, substrate in the waters, and ice cubes that use for operating Minitrawl. The result of this research showed value TPC heterotrophic bacteria (TSA) which contaminated sample ITE (fish wihthout ice) 9,89 Log CFU/ml, IS (fish with ice) 9,85 Log CFU/ml, AL (sea water) 4,91 Log CFU/ml, S (substrate in the waters) 5,28 Log CFU/ml and ES (ice cubes that use for fishermen) 3,29 Log CFU/ml amount of pathogenic bacteria (TCBS) in ITE 5,27 Log CFU/ml , IS 3,24 Log CFU/ml, AL 3,45 Log CFU/ml, S 1,78 Log CFU/ml, ES 3,87 Log CFU/ml,most highest amount is in the ITE with value of TPC heterotrophic bacteria (TSA) 9,89 Log CFU/ml and pathogenic bacteria (TCBS) 5,27 Log CFU/ml. Ikan merupakan sumber protein hewani yang umumnya menjadi pilihan masyarakat karena memiliki harga yang relatif murah dan mudah didapat. Ikan yang dijual dalam kondisi telah mati rentan terhadap penurunan kualitasnya sehingga bakteri dapat dengan mudah berkembang biak. Data tentang kelimpahan bakteri pada ikan sehubungan dengan jenis alat tangkap perlu menjadi perhatian dengan mempertimbangkan metode penangkapan yang berbeda antara satu alat tangkap dengan alat tangkap lainnya yang sangat mungkin mempengaruhi kualitas hasil tangkapannya. Oleh karena itu, penelitian dilakukan dengan menggunakan MiniTrawl. Tujuan penelitian adalah untuk mengetahui kelimpahan bakteri pada ikan yang ditangkap menggunakan MiniTrawl sebelum diberi es dan sesudah diberi es dengan mempertimbangkan kelimpahan bakteri di substrat perairan dan es batu yang digunakan nelayan. Metode penelitian TPC (Total Plate Count) menggunakan media Tryptic Soy Agar (TSA) dan bakteri patogen menggunakan media Thiosulfate Citrate Bile Salt Sucrose Agar (TCBS) pada ikan kardinal pita lebar (Apogon fasciatus), air laut, substrat perairan dan es batu yang digunakan pada pengoperasian MiniTrawl. Hasil penelitian menujukkan nilai TPC bakteri heterotrof (TSA) yang mengkontaminasi sampel ITE (ikan tanpa es) 9,89 Log CFU/ml, IS (ikan dengan es) 9,85 Log CFU/ml, AL (air laut) 4,91 Log CFU/ml, S (substrat perairan) 5,28 Log CFU/ml dan ES (es batu yang digunakan nelayan) 3,29 Log CFU/ml. Kelimpahan bakteri patogen (TCBS) pada ITE 5,27 Log CFU/ml, IS 3,24 Log CFU/ml, AL 3,45 Log CFU/ml, S 1,78 Log CFU/ml, dan ES 3,87 Log CFU/ml. Kelimpahan tertinggi yaitu pada ITE dengan nilai TPC bakteri heterotrof (TSA) 9,89 Log CFU/ml dan bakteri patogen (TCBS) 5,27 Log CFU/ml.
Biodegradation is an alternative in overcoming oil pollution biologically. Utilization of bacteria obtained from contaminated (indigenous) areas is known to be more effective in the degradation process. This study aims to determine the characteristics of indigenous bacteria that have been isolated from Kamal harbor water samples, and the consortium will be compared with the consortium of bacteria isolated from salt waste water (bittern) samples to determine their ability to degrade diesel pollutants. The method used in this research is the enrichment method, to obtain bacterial isolates. The consortium was treated with solar concentrations of 1%, 1.5%, 2%, 2.5%, and 3%. The results showed that 4 types of bacteria were obtained (BI.1, BI.2, BI.3, and BI. 4) from Kamal harbor waters with different macroscopic and microscopic characteristics. The highest %total petroleum hydrocarbons (TPH) reduction by the indigenous consortium was in the 3% diesel treatment with a value of 3.87, while in the bittern consortium there was 2.5% diesel treatment with a value of 3.34. Biodegradasi merupakan salah satu alternatif dalam menanggulangi pencemaran minyak secara biologis. Pemanfaatan bakteri yang diperoleh dari area pencemaran (indigenous)diketahui akan lebih efektif dalam proses degradasi. Penelitian ini bertujuan untuk mengetahui karakteristik bakteri yang berhasil diisolasi dari sampel air pelabuhan kamal, dan konsorsiumnya akan dibandingkan dengan konsorsium bakteri yang berhasil diisolasi dari sampel air limbah garam (bittern) guna mengetahui kemampuannya dalam mendegradasi bahan pencemar solar. Metode yang digunakan dalam penelitian yaitu metode enrichment, untuk memperoleh isolat bakteri. Konsorsium diberi perlakuan dengan konsentrasi solar 1%, 1,5%, 2%, 2,5%, dan 3%. Hasli penelitian menunjukkan diperoleh 4 jenis bakteri (BI.1, BI.2, BI.3, dan BI.4) dari perairan pelabuhan kamal dengan karakteristik makroskopis dan mikroskopis yang berbeda-beda. Penurunan %total petroleum hidrokarbon (TPH) tertinggi oleh konsorsium indigenous terdapat pada perlakuan solar 3% dengan nilai sebesar 3,87, sedangkan pada konsorsium bittern terdapat pada perlakuan solar 2,5% dengan nilai sebesar 3,34.
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