Biomineralization is a process by which living organisms produce minerals. The extracellular production of these biominerals by microbes has potential for various bioengineering applications. For example, crack remediation and improvement of durability of concrete is an important goal for engineers and biomineral-producing microbes could be a useful tool in achieving this goal. Here we report the isolation, biochemical characterization and molecular identification of Pseudomonas azotoformans, a microbe that produces calcite and which potentially be used to repair cracks in concrete structures. Initially, 38 bacterial isolates were isolated from soil and cements. As a first test, the isolates were screened using a urease assay followed by biochemical tests for the rate of urea hydrolysis, calcite production and the insolubility of calcite. Molecular amplification and sequencing of a 16S rRNA fragment of selected isolates permitted us to identify P. azotoformans as a good candidate for preparation of biotechnological concrete. This species was isolated from soil and the results show that among the tested isolates it had the highest rate of urea hydrolysis, produced the highest amount of calcite, which, furthermore was the most adhesive and insoluble. This species is thus of interest as an agent with the potential ability to repair cracks in concrete.
Autoclaved Aerated Concrete (AAC) is also being produced for many years, there are still some points that need to be clarified. One of these points needs to know is humidity intrusion effects on AAC members in areas with high relative humidity levels of Mediterranean climates which are important in durability and insulation properties of AAC. Therefore, some tests on mechanical and physical properties of ACC concrete carried out. These include thermal insulation and fire resistance tests under different level of humidity ACC blocks. According to the test results; increasing in humidity condition inside the chamber during heating procedure under steady state condition, caused increasing in average temperature change on outside surface of AAC wall. AAC losses its mass and mechanical properties subjected to the high elevated temperature above 500°C.
Progressive collapse is a relatively rare event, as it requires both an abnormal loading to initiate the local damage and a structure that lacks adequate continuity, ductility and redundancy to resist the spread of damage. However, significant casualties can result when collapse occurs. Heavy impact loads due to tsunami against building can be one of the scenarios of progressive collapse during tsunami disaster. Since progressive collapse includes material and geometry nonlinearity during collapse propagation, in the present research capability of 2 models for the material nonlinearity in simulating actual behavior of structures during collapse is compared with recent experimental results of a Reinforced Concrete (RC) frame. The results demonstrate that a material nonlinearity model, that is based on the idealized component load-deformation behavior, is not a proper representation for the real behavior of structures during progressive collapse and is so conservative.
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