Mesenchymal stromal cells (MSCs) have stimulated much interest in the scientific community. Mouse MSCs serve as an ideal tool to explore cell biology and therapeutic potential of MSCs. Therefore, establishment of optimal, standardized protocol for mouse MSCs isolation and culture is required. Our aim was to develop and describe an efficient, reliable, and easy-to-perform protocol for isolation and culture of mouse bone marrow mesenchymal stromal cells MSC(M). Our protocol is based on a combination of flushing method and mechanical crushing of the bones. MSC(M) isolated using our protocol showed spindle-shaped appearance, positive expression of CD73 and CD44 markers, weak expression of CD34 and CD105, and negative expression for CD11b. They were also able to differentiate into mesodermal lineages such as adipocytes, and osteocytes. We hope that the data presented in this paper are of practical importance and can be used in clinical and research applications, and cell banking.
Mesenchymal stromal cell-derived conditioned medium therapy is a rapidly developing field of research that seems to have a significant impact on the treatment of various diseases. In the present study, the therapeutic effect of conditioned medium derived from bone marrow mesenchymal stromal cells (BMSCs) co-cultured with hepatocytes (H/BMSCs CM) was investigated for alleviation of CCl 4 -induced liver damage in mice.
Materials and methodsHepatocytes were co-cultured with BMSCs for 20 h. H/ BMSCs CM were then collected. Mice were intraperitoneally injected with CCl 4 twice a week for 7 weeks, followed by injection of H/BMSCs CM. Four weeks after the CM treatment, serum levels of ALT, AST, alkaline phosphatase (ALP), and albumin were determined, liver histopathology was examined, and ELISA assay was performed to measure the serum levels of IL-4 and IFN-γ.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.