Polygalacturonases are important enzymes used in industrial applications that treated plant material like Juice extraction, Textile, Clarification etc. Fungi from genus Aspergillus are one of the most important sources of this enzyme. We production Polygalacturonase by submerged fermentation using seaweed waste. Five PG P1-P5 were differentiated on DEAE-Sepharose column. The homogeneity of PGP2a was identified on Sephacryl S-200 chromatography. PGP2a had a molecular weight of 20 kDa by Sephacryl S-200 and SDS-PAGE. The enzyme had an optimal pH of 6 also the temperature optimal of PGP2a was 40 °C. The thermal stability of PGP2a was detected up to 50 °C and the enzyme was highly stable till 60 °C after 30min incubation. The V max and K m values of PGP2a were 4.27 mg/ml and 1.16µmol min -1 mg-1 , respectively. The metal ions except only Co 2+ and Hg 2+ was found to enhance the PGP2a activity.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.