The universal emphasis on the study of green nanotechnology has led to biologically harmless uses of wide-ranged nanomaterials. Nanotechnology deals with the production of nanosized particles with regular morphology and properties. Various researches have been directed on nanomaterial synthesis by physical, chemical, and biological means. Understanding the safety of both environment and in vivo, a biogenic approach particularly plant-derived synthesis is the best strategy. Silver-zinc oxide nanoparticles are most effective. Moreover, these engineered nanomaterials via morphological modifications attain improved performance in antimicrobial, biomedical, environmental, and therapeutic applications. This article evaluates manufacturing strategies for silver-zinc oxide nanoparticles via plant-derived means along with highlighting their broad range of uses in bionanotechnology.

Chitinases are responsible for catalyzing the hydrolysis of chitin and contribute to plant defense against fungal pathogens by degrading fungal chitin. In this study, genome-wide identification of the chitinase gene family of wild apple (Malus sieversii) and domesticated apple (Malus domestica) was conducted, and the expression profile was analyzed in response to Valsa mali infection. A total of 36 and 47 chitinase genes belonging to the glycosyl hydrolase 18 (GH18) and 19 (GH19) families were identified in the genomes of M. sieversii and M. domestica, respectively. These genes were classified into five classes based on their phylogenetic relationships and conserved catalytic domains. The genes were randomly distributed on the chromosomes and exhibited expansion by tandem and segmental duplication. Eight of the 36 MsChi genes and 17 of the 47 MdChi genes were differentially expressed in response to V. mali inoculation. In particular, MsChi35 and its ortholog MdChi41, a class IV chitinase, were constitutively expressed at high levels in M. sieversii and domesticated apple, respectively, and may play a crucial role in the defense response against V. mali. These results improve knowledge of the chitinase gene family in apple species and provide a foundation for further studies of fungal disease prevention in apple.

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Plant surfaces are covered with cuticle wax and are the first barrier between a plant and environmental stresses. Eceriferum (CER) is an important gene family involved in wax biosynthesis and stress resistance. In this study, for the first time, 34 CER genes were identified in the passion fruit (Passiflora edulis) genome, and PeCER proteins varied in physicochemical properties. A phylogenetic tree was constructed and divided into seven clades to identify the evolutionary relationship with other plant species. Gene structure analyses revealed that conserved motifs ranged from 1 to 24, and that exons ranged from 1 to 29. The cis-element analysis provides insight into possible roles of PeCER genes in plant growth, development and stress responses. The syntenic analysis revealed that segmental (six gene pairs) and tandem (six gene pairs) gene duplication played an important role in the expansion of PeCER genes and underwent a strong purifying selection. In addition, 12 putative ped-miRNAs were identified to be targeting 16 PeCER genes, and PeCER6 was the most targeted by four miRNAs including ped-miR157a-5p, ped-miR164b-5p, ped-miR319b, and ped-miR319l. Potential transcription factors (TFs) such as ERF, AP2, MYB, and bZIP were predicted and visualized in a TF regulatory network interacting with PeCER genes. GO and KEGG annotation analysis revealed that PeCER genes were highly related to fatty acid, cutin, and wax biosynthesis, plant-pathogen interactions, and stress response pathways. The hypothesis that most PeCER proteins were predicted to localize to the plasma membrane was validated by transient expression assays of PeCER32 protein in onion epidermal cells. qRT-PCR expression results showed that most of the PeCER genes including PeCER1, PeCER11, PeCER15, PeCER17, and PeCER32 were upregulated under drought and Fusarium kyushuense stress conditions compared to controls. These findings provide a foundation for further studies on functions of PeCER genes to further facilitate the genetic modification of passion fruit wax biosynthesis and stress resistance.

Plants may experience adverse effects from Cadmium (Cd). As a result of its toxicity and mobility within the soil-plant continuum, it is attracting the attention of soil scientists and plant nutritionists. In this study, we subjected young Eruca sativa Mill. seedlings to different levels of Cd applications (0, 1.5, 6 and 30 µmol/L) via pot experiment to explore its morpho-physio-biochemical adaptations. Our results revealed a significant Cd accumulation in leaves at high Cd stress. It was also demonstrated that Cd stress inhibited photosynthetic rate and pigment levels, ascorbate peroxidase (APX), guaiacol peroxidase (GPX), catalase (CAT), and superoxide dismutase (SOD) enzyme activities, and increased malondialdehyde (MDA) levels. Conversely, the concentration of total ascorbate (TAS) increased at all levels of Cd application, whereas that of ascorbic acid (ASA), and dehydroascorbate (DHA) increased at 1.5 (non-significant), 6, 30 and 6 µmol/L (significant), though their concentrations decreased non-significantly at 30 µmol/L application. In conclusion, Cd-subjected E. sativa seedlings diverted much energy from growth towards the synthesis of anti-oxidant metabolites and osmolytes. However, they did not seem to have protected the E. sativa seedlings from Cd-induced oxidative stress, causing a decrease in osmotic adjustment, and an increase in oxidative damage, which resulted in a reduction in photosynthesis and growth. Accordingly, we recommend that the cultivation of E. sativa should be avoided on soil with Cd contamination.

It has been shown that jasmonic acid (JA) can alleviate drought stress. Nevertheless, there are still many questions regarding the JA-induced physiological and biochemical mechanisms that underlie the adaptation of plants to drought stress. Hence, the aim of this study was to investigate whether JA application was beneficial for the antioxidant activity, plant performance, and growth of Grewia asiatica L. Therefore, a study was conducted on G. asiatica plants aged six months, exposing them to 100% and 60% of their field capacity. A JA application was only made when the plants were experiencing moderate drought stress (average stem water potential of 1.0 MPa, considered moderate drought stress), and physiological and biochemical measures were monitored throughout the 14-day period. In contrast to untreated plants, the JA-treated plants displayed an improvement in plant growth by 15.5% and increased CO2 assimilation (AN) by 43.9% as well as stomatal conductance (GS) by 42.7% on day 3. The ascorbate peroxidase (APX), glutathione peroxidase (GPX), and superoxide dismutase (SOD) activities of drought-stressed JA-treated plants increased by 87%, 78%, and 60%, respectively, on day 3. In addition, G. asiatica plants stressed by drought accumulated 34% more phenolics and 63% more antioxidants when exposed to JA. This study aimed to understand the mechanism by which G. asiatica survives in drought conditions by utilizing the JA system.

Pesticides have great potential to contaminate resources of drinking water by percolating and leaching, when applied in the agriculture sector as well as in domestic region. Activated carbon (AC) and Biochar (BCH) were used for adsorption in a fixed-bed column system. Both of the adsorbent-packed columns indicated an increase in the breakthrough time for atrazine from 3350 to 5800 min and 3200 to 5700 min, chlorothalanil 3200–5600 min and 3150–5550 min, β-endosulfan 3050–5400 min and 2950–5400 min, and α-endosulfan 2900–5200 min and 2850–5200 min with bed heights from 10 cm to 15 cm, respectively. Similarly, when flow rate increased from 0.5 to 1.5 mL min−1 and contaminant concentration from 50–100 µg L−1, it resulted in a decrease in exhaust time. The models of Yoon–Nelson (R2 = 0.9427) and Thomas (R2 = 0.9921) describe the process of adsorption to be best well-under optimal conditions. Both the adsorbents would be efficiently utilized as the best adsorbents to remediate pesticide-contaminated water under optimal conditions. Pesticides adsorption onto adsorbents followed the order of atrazine > chlorothalanil > β-endosulfan > α-endosulfan.

Extreme heat, droughts, pests, diseases, and short bursts of heavy rain make potato production unsustainable. This unfavorable environment negatively affects potato productivity and yield levels. Within the next few years, conditions will likely deteriorate even more. In potato cultivation, straw mulching has been shown to increase yields by promoting the growth of beneficial bacteria in the soil. Mulching improves soil humidity, decreases transpiration, and cools the soil in dry and hot regions. There is a global decline in potato yields per hectare due to poor nutrient management, moderately humid years, and high disease pressure caused by Phytophthora infestans and Alternaria species. Farmers must take cultivation measures to achieve economic efficiency and adequate yields. A range of practices contributes to better potato yields and productivity, such as the use of appropriate fungicides, planting high-yielding varieties, and increasing row spacing. These practices complicate cultivation and affect profits. Furthermore, inorganic nitrogen in the soil regularly causes acidification, eroding soil fertility. As a result of land preparation, straw residues from rice and maize are collected from the field and destroyed or burned, which depletes nutrients and pollutes the air. Returning these residues to the soil, however, can improve its quality. Integrating rice and maize straw mulching into potato cultivation practices can enhance agricultural sustainability, productivity, and yield. This review will focus on using rice and maize straw mulching in cultivating potatoes. Straw mulching promotes sustainable potato growth, increasing productivity and quality while minimizing reliance on chemical inputs. Such practices can mitigate the need for synthetic fertilizers to enhance sustainable agriculture, ensure long-term growth, improve soil health, increase yields, and promote sustainable agriculture.