Several studies included our preceding works on different types and doses of plasma sources on both normal and cancerous cells. All previous research tried to discuss and interpret cancer treatment concepts given plasma and cell interaction. Plasma has an effective role and specification character on cancerous cells outcome via many investigations (genetically, immunologically, and biochemically measurements) lead us to numerous paths of different theories which may create a new approach for considerations. This study was designed using a plasma jet powered by a Tesla coil used for generating Cold Atmospheric Pressure Plasma Jet (CAPPJ) from dielectric barrier discharge. The subject samples were categorized into three groups, the first was the cancer cell line. The second was normal blood samples whereas, the third one was non-exposed blood cells cultivated in CAPPJ-exposed cultures. The Cytokinesis Blocked Micronucleus Test (CBMN), a cytome assay, the protein expression of the P53 and Bcl2 genes, the interleukins (IL-1β, IL-6, and IL-10), and tumor necrosis factor (TNF-α) were the variables used in the current investigation. Results indicated that the direct interaction between cells and CAPPJ is more efficient than cells cultivated in CAPPJ-exposed cultures. Cell viability and protein expression levels of Bcl2 and P53 genes in CAPPJ irradiated Breast Cancer Cell lines (BCC) were remarkably valuable. CAPPJ affects cells via not only free radicals and enhancement of several important pathways but may be via direct interaction with DNA.
Vitiligo is a multifactorial disorder disease characterized by loss of functional melanocytes, genetic damage, excess inflammation, autoimmune dysfunction, and extreme oxidative stress in lesions. Unfortunately, there is not enough response to obtainable therapies nowadays. The present study was designed using a plasma jet powered by a Tesla coil, which can be used for generating cold atmospheric pressure plasma jet (CAPPJ) from dielectric barrier discharge. The subject samples were categorized into two groups, first one was normal blood samples (control) and the second was vitiligo patient's blood samples. Present study parameters were as a following: cytokinesis blocked micronucleus test cytome assay, protein expression of both P53 and Bcl2 genes, interleukins (IL-1β, IL-6, and IL-10) and tumor necrosis factor-alpha. The study plans to investigate the effect of CAPPJ on whole blood cultures of vitiligo patients compared with matched control blood cultures. CAPPJ affects white blood cells (lymphocytes) and reduces its production of inflammatory cytokines, which cascade the autoimmune response. In addition, CAPPJ successfully reduces Mni frequencies, enhancing apoptosis and normalizing P53 and Bcl2 genes expression in vitiligo samples. The current study showed that CAPPJ exposure can achieve enhanced treatment of some types of autoimmune diseases such as vitiligo through molecular mechanisms of action, cell cycle regulation, activation of cell signaling pathways, and/or pleiotropic antioxidant and anti-inflammatory properties.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.