Two field experiments were conducted during 2012 and 2013 seasons in the Experimental Farm of Agriculture Research Station, Agric. Res. Center at Ismailia, Egypt to study the effect of four levels of phosphatic fertilizer i.e., 15.5, 23.25 and 31.0 kg P 2 O 5 /fad (faddan= 4200 m2) with and without the bio fertilizers phosphorine or microbein on yield, yield components and yield quality of two peanut varieties, Giza 6 as erect cv and Gereogry as spread cv. The spread variety Gereogry surpassed significantly the erect variety Giza 6 in yield (pods, seeds and biological yield/fad), yield components (plant height, number and dry weight of pods/plant, dry weight of seeds /plant, 100-seed weight and shelling percentage) and yield quality (seed oil content% and oil and protein yield/fad. While, the two studied varieties did not differ significantly from each other in dry weight of straw/plant, fodder yield/fad and seed protein content %). Increasing phosphorus levels from 0 to 31 kg P 2 O 5 /fad increased significantly the forementioned yield, yield components and yield quality; expect the non significant effect of P levels on seed protein content %. It is worthy to note that bio fertilizers phosphorine or microbein increased the efficiency of peanut plants in utilizing phosphours fertilizer, which in turn had favourable effect on peanut productivity. The increment of yield, yield component and yield quality of peanutcvs was more pronounced and its values were higher when peanut plants fertilized with phosphorus fertilizer combined with phosphorine or microbein than fertilized with P only and the two biofertilizers did not differ significantly in this respect. P levels up to the highest level (31 kg P 2 O 5 ) with or without biofertilizers increased significantly peanut yield, yield components and yield quality, except that there was no significant difference between to two higher levels of P (23.25 and 31.0 kg P 2 O 5 /fad) in plant height, 100-seed weight, pods yield/fad, seed oil content % and protein yield/fad.