Bacterial nanocellulose (Bnc) has been drawing enormous attention because of its versatile properties. Herein, we shed light on the BNC production by a novel bacterial isolate (MD1) utilizing various agroindustrial wastes. Using 16S rRNA nucleotide sequences, the isolate was identified as Komagataeibacter saccharivorans MD1. For the first time, BNC synthesis by K. saccharivorans MD1 was investigated utilizing wastes of palm date, fig, and sugarcane molasses along with glucose on the Hestrin-Schramm (HS) medium as a control. After incubation for 168 h, the highest BNC yield was perceived on the molasses medium recording 3.9 g/L with an initial concentration of (v/v) 10%. The physicochemical characteristics of the BNC sheets were inspected adopting field-emission scanning electron microscope (FESEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) analysis. The FESEM characterization revealed no impact of the wastes on either fiber diameter or the branching scheme, whereas the AFM depicted a BNC film with minimal roughness was generated using date wastes. Furthermore, a high crystallinity index was estimated by XRD up to 94% for the date wastes-derived BNC, while the FTIR analyses exhibited very similar profiles for all BNC films. Additionally, mechanical characteristics and water holding capacity of the produced Bncs were studied. Our findings substantiated that expensive substrates could be exchanged by agro-industrial wastes for Bnc production conserving its remarkable physical and microstructural properties. In the last decades, bacterial nanocellulose (BNC) has earned increasing global interest because of its remarkable physical and chemical properties, including green processing, low production costs, elevated mechanical properties, hydrophilicity, excellent biocompatibility, and biodegradability 1,2. Certain gram-negative non-pathogenic bacterial genera like Rhizobium, Xanthococcus, Pseudomonas, Azotobacter, Aerobacter, and Alcaligenes were reported to produce nanocellulose extracellularly, but the most common BNC-producing strains belong to the genus Komagataeibacter (formerly Acetobacter or commonly acetic acid bacteria) 1. Bacteria produce the BNC through a process of dual coupled steps: polymerization and crystallization. In the bacterial cytoplasm, glucose residues polymerize to β-1,4 glucan linear chains where they are extracellularly secreted. The developed chains are crystallized to microfibrils, then certain numbers of microfibrils consolidate to materialize highly pure 3D porous network of entangled nanoribbons of 20-60 nm in width 3 .
