Barada River is characterized by an abundant growth of freshwater algae. Algae have been emerged as a new source for bioactive compounds, in this research Cladophora crispata was cultivated in the outdoor method, and algae sulfated Polysaccharides (SPs) were extracted by ultrasonic-assisted extraction methods. After extraction, Gel filtration was used to purify the crude SPs; SPs compounds were determined and selected and we investigated the effect of SPs as antibacterial agents. the purified extract gave two fractions (F1 and F2). The chemical components of both crude and purified SPs were then determind. The highest carbohydrate content (74.12%) and protein content (4.02%) was found in the crude extract, while the highest sulfate content (12.17%) was found in F2, and the highest uronic content (18.46%) was found in F1. Fourier transforms infrared Spectroscopy (FT-IR) was used for confirmed that the crud and fractions consist of sugar, uronic acids, protein unite, and sulfate groups. Both F1, and F2 was found to consist of rhamnose, galactose, xylose, and ribose based on the High performance liquid chromatography (HPLC) separation. Each fraction showed an inhibitory effect on the gram-positive and gram-negative bacteria. F2 has the lowest minimum inhabit concentration (MIC) value against Enterobacter aerogenes and Staphylococcus aureus; where its MIC values were 28 and 10 (mg/ml) respectively. Algae polysaccharides had the most important interest due to their antibacterial properties, which is makes them included in pharmacy and food applications.
Barada River is characterized by an abundant growth of freshwater algae. Algae have been emerged as a new source for bioactive compounds, in this research Cladophoracrispata was cultivated in the outdoor method, and algae sulfated Polysaccharides (SPs) were extracted by ultrasonic-assisted extraction methods. After extraction, Gel filtration was used to purify the crude SPs; SPs compounds were determined and selected and we investigated the effect of SPs as antibacterial agents. the purified extract gave two fractions (F1 and F2). The chemical components of both crude and purified SPs were then determind. The highest carbohydrate content (74.12%) and protein content (4.02%) was found in the crude extract, while the highest sulfate content (12.17%) was found in F2, and the highest uronic content (18.46%) was found in F1. Fourier transforms infrared Spectroscopy (FT-IR) was used for confirmed that the crud and fractions consist of sugar, uronic acids, protein unite, and sulfate groups. Both F1, and F2 was found to consist of rhamnose, galactose, xylose, and ribose based on the High performance liquid chromatography (HPLC) separation. Each fraction showed an inhibitory effect on the gram-positive and gram-negative bacteria. F2 has the lowest minimum inhabit concentration (MIC) value against Enterobacteraerogenes and Staphylococcusaureus; where its MIC values were 28 and 10 (mg/ml) respectively. Algae polysaccharides had the most important interest due to their antibacterial properties, which is makes them included in pharmacy and food applications.
Barada River is characterized by an abundant growth of freshwater algae. Algae have been emerged as a new source for bioactive compounds, in this research Cladophoracrispata was cultivated in the outdoor method, and algae sulfated Polysaccharides (SPs) were extracted by ultrasonic-assisted extraction methods. After extraction, Gel filtration was used to purify the crude SPs; SPs compounds were determined and selected and we investigated the effect of SPs as antibacterial agents. the purified extract gave two fractions (F1 and F2). The chemical components of both crude and purified SPs were then determind. The highest carbohydrate content (74.12%) and protein content (4.02%) was found in the crude extract, while the highest sulfate content (12.17%) was found in F2, and the highest uronic content (18.46%) was found in F1. Fourier transforms infrared Spectroscopy (FT-IR) was used for confirmed that the crud and fractions consist of sugar, uronic acids, protein unite, and sulfate groups. Both F1, and F2 was found to consist of rhamnose, galactose, xylose, and ribose based on the High performance liquid chromatography (HPLC) separation. Each fraction showed an inhibitory effect on the gram-positive and gram-negative bacteria. F2 has the lowest minimum inhabit concentration (MIC) value against Enterobacteraerogenes and Staphylococcusaureus; where its MIC values were 28 and 10 (mg/ml) respectively. Algae polysaccharides had the most important interest due to their antibacterial properties, which is makes them included in pharmacy and food applications.
Barada River is characterized by an abundant growth of freshwater algae. In light of the increased interest in algae to find new sources of bioactive compounds, this research, aimed to extract sulfated Polysaccharides (SPs) from freshwater green algae Cladophoracrispata using ultrasonic-assisted extraction methods after cultivates it by the outdoor method. Also we determined and selected its compounds,and we investigation effect SPs as antibacterial agent After extraction, Sephadex gel G-100 was used for the Purification of SPs, where the purifcation gave two fractions, namley F1 and F2. The chemical characterization of crud SPs and its fractions were investigated. The highest carbohydrate and protein contents were found in crude SPs with the ratios of 74.12%, and 4.02%, respectively, while the highest sulfate content 12.17% was found in fraction 2, and the higest uronic content 18.46% was found in fraction 1. Fourier transforms infrared Spectroscopy (FT-IR) used for confirmed the crud and fractions consist of sugar, uronic acids, protein unite, and sulfate groups. Both F1, and F2 consist of rhamnose, galactose, xylose, and ribose based on separation on the High performance liquid chromatography (HPLC). Each fraction has inhibitory effect on bacteria negative and positive gram. F2 have the lowest minimization inhabit concentration (MIC) value against Enterobacteraerogenes and Staphylococcusaureus; where MIC were 28 and 10 mg/ml respectively. Polysaccharides had the most important interest because they have antibacterial properties, making them inter in pharmacy and food applications.
Graphical Abstract Sulfated polysaccharides (SPs) from Cladophora crispata were extracted by ultrasonic bath: purify the crude SPs. Compounds of SPs were identified, and we investigated the effect of SPs as antibacterial agents.
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