Green synthesis of nanoparticles is considered an efficient method when compared with chemical and physical methods because of its bulk production, eco-friendliness and low cost norms. The present study reports, for the first time, green synthesis of silver nanoparticles (AgNPs) at room temperature using fruit extract. The visual appearance of brownish colour with an absorption band at 450 nm, as detected by ultraviolet-visible spectrophotometer analysis, confirmed the formation of AgNPs. X-ray diffraction confirmed the AgNPs to be crystalline with a face-centred lattice. The transmission electron microscopy-energy dispersive X-ray spectroscopy image showed the AgNPs are poly-dispersed and are mostly spherical and oval in shape with particle size ranging from 2 to 40 nm. Furthermore, Fourier transform-infrared spectra of the synthesised AgNPs confirmed the presence of phytoconstituents as a capping agent. The antimicrobial activity study showed that the AgNPs exhibited high microbial activity against susp. , and . The highest antimicrobial activity of AgNPs synthesised by fruit extract was observed in , susp. and with zone of inhibition, 26.67 mm.
In the present years, nanobiotechnology has to turn out to be an important division of research. Here, this work reported a novel Mikania micrantha leaf extract-mediated silver nanoparticle (AgNP) under room temperature condition. The biosynthesised AgNPs were further characterised by various analysis techniques such as ultraviolet-visible spectrophotometer, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and transmission electron microscope (TEM) energy-dispersive X-ray spectroscopy. The formation of AgNPs was confirmed by XRD spectrum which displayed intense peaks corresponding to the (1 1 1), (2 0 0), (2 2 0) and (3 1 1) lattice planes of Ag. The plant extracts stirred with Ag nitrate solution gradually changes its colour from light yellow to dark brown. The biogenic AgNPs were mostly spherical in shape with varying sizes ranging from 5 to 20 nm, as evident by TEM analysis. FT-IR spectra of the extract revealed the presence of amide I and amide II bonds of the proteins. The synthesised AgNPs showed excellent activity results against various Gram-positive and Gram-negative bacterial strains.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.