Medicinal plants have bioactive compounds which are used for curing of various human diseases and also play an important role in healing. Secondary constituents contain alkaloids, flavonoids, phenol, saponin, steroids and tannins. Medicinal plants have anticancer, antimicrobial, antidiabetic, antidiuretic and anti- inflammation activities ect. The increasing interest in powerful biological activity of secondary metabolites outlined the necessity of determining their contents in medicinal plants. Lam () is a fast-growing and drought-resistant tree of the family. The tree is known with some common names: miracle, ben oil, drumstick, horseradish or simply moringa. Phytochemical studies of plant organs showed the plant as a rich source of primary and secondary metabolites belonging to different classes of organic compounds. Pharmacological studies confirmed the use of the plant to cure several diseases and to possess nutraceutical properties. The objective of this study was to investigate pharmacognostical, phytochemical features and antioxidant activity of hydroalcoholic extracts of leaves by using DPPH assay method. The different pharmacognostical parameters were evaluated as per standard protocols with some modifications. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. The antioxidant activity of hydroalcoholic extract of the leaves was assessed against DPPH method using standard protocols.Phytochemical analysis revealed the presence of alkaloids, glycosides, flavonoids, tannins, amino acid and carbohydrate. The total phenolic and flavonoids content of leaves of hydroalcoholic extract was 0.864and 1.014 mg/100mg respectively. The activities of hydroalcoholic leaves extract against DPPH assay method were concentration dependent with IC values of ascorbic acid and extracts 17.68 and 79.10µg/ml respectively. The present study concluded that the crude extract of is a rich source of secondary phytoconstituents which impart significant antioxidant potential. The findings of the present study will be helpful to phytochemists, pharmacologists and pharmaceutical industries.
Plants have served human beings as a natural source for treatments and therapies from ancient times, amongst them medicinal herbs have gain attention because of its wide use and less side effects. In the recent years plant research has increased throughout the world and a huge amount of evidences have been collected to show immense potential of medicinal plants used in various traditional systems. Hordeum vulgare Linn seeds (H. vulgare, Commonly known as barley, Family: Poaceae), is an erect annual herb, 50 to 100 cm high, cultivated in the plains as well as in the hilly region of Himalaya, up to an altitude of 4000 m, in Indo- Gangetic area and Madhya Pradesh. It is locally called Jav and its seed used by traditional medical practitioners in the treatment of many diseases including liver diseases. Seeds are useful in vitiated conditions of kapha and pitta, asthma, fever, bronchiotitis, urocystitis, urethritis, gastric disorders, ulcers and anemia etc. The objective of this study was to investigate pharmacognostical, phytochemical features and antioxidant activity of hydroalcoholic extracts of H. vulgare seed by using DPPH assay method. The different pharmacognostical parameters were evaluated as per standard protocols with some modifications. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. The in vitro antioxidant activity of hydroalcoholic extract of the seed was assessed against DPPH method using standard protocols. Phytochemical analysis revealed the presence of alkaloids, glycosides, flavonoids, steroids, saponins and carbohydrate. The total phenolic and flavonoids content of H. vulgare seed of hydroalcoholic extract was 0.693 and 0.497mg/100mg respectively. The activities of hydroalcoholic leaves extract against DPPH assay method were concentration dependent with IC 50 values of ascorbic acid and extracts 27.82 and 76.92μg/ml respectively. These studies provided information for correct identification of this plant material. The diverse array of phytochemicals present in the plant thus suggests its therapeutic potentials which may be explored in drug manufacturing industry as well as in traditional medicine.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.