Dermatophytoses are a group of superficial fungal infections of keratinized tissues. Though they do not cause mortality they cause morbidity and pose a major public health problem especially in tropical countries like India. The present study is done to know the prevalence of dermatophytes and to identify the causative agents in Hyderabad area. A total of 180 samples were collected for a period of 1 year from infected skin, hair, and nails. Samples were collected under aseptic conditions and were then subjected to direct microscopy by KOH mount and culture on SDA. Dermatophytes were seen more in males (126/180). Direct microscopy by KOH mount revealed presence of fungal elements in (144/180) of samples. T.mentagrophytes was the most predominant isolate followed by T.rubrum and T.violaceum. Dermatophytic infections are worldwide in distribution with superficial mycoses being more prevalent in India where heat and moisture play an important role. Dermatophytosis has been reported throughout the year in the present study locality and an increase in prevalence of certain species was found.

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Methicillin resistant Staphylococcus aureus (MRSA) is a growing concern in the community and in hospitals ever since it has been diagnosed. As staphylococcus has been the dominant pathogen of wounds, Methicillin resistance made the treatment a tough challenge to the clinician. The growing problem of antibiotic resistance and the need for alternative treatments have led to a reappearance of importance in phage therapy. Scope: The present study aimed on MRSA phage types and its antibiotic susceptibility pattern in wounds. Material and Methods: In this study a total of 92 coagulase positive Staphylococcus aureus obtained from various wounds are identified by inoculating on Blood agar, MacConkey agar and Nutrient agar and processed further by necessary biochemical tests and tube coagulase tests. All strains were tested for Methicillin resistance and phage typing. Findings: The results obtained were MRSA (22) of 23.92%, Methicillin sensitive staphylococcus aureus (MSSA) (70) of 76.08% and the predominant phage type in MRSA and MSSA obtained was phage III. The typable MRSA strains and MSSA strains showed 100% sensitivity to Vancomycin followed by 89.13% sensitivity to Cefaperazone-Sulbactum, 87.5% to Ciprofloxacin. Conclusion: Phage typing may be a very useful diagnostic method in future.

In cancer patients, infection is the most significant and continuous problem. This study was done to show the spectrum of bacteria and sites of isolation in febrile neutropenic cancer patients. A 1-year study of all isolates was conducted from various sites in our hospital. All the samples were processed, and isolates were identified as per CLSI guidelines. The commonest organism isolated was Escherichia coli among Gram negative organisms and Staphylococcus aureus among Gram positive organism. Out of 76 isolates 49 were Gram negative and 27 were Gram positive. The pattern of infectious agents has changed in neutropenic patients over time and this postulates the need of other studies to give the most up to date insight of the causative organism to the physician.